Alterations of the (/sup 59/Fe)ferric citrate biodistribution in hyperferremic mice after the administration of pyrophosphate and desferrioxamine
One of the most efficient anions in enhancing the ability of desferrioxamine (DFO) to remove iron from transferrin in vitro has been shown to be pyrophosphate (PYP). To evaluate the in vivo effect of PYP in hyperferremic mice, the biodistribution of (/sup 59/Fe)ferric citrate was studied after the i.p. administration of: 1) only saline in the control animals; 2) an aqueous solution of tetrasodium diphosphate (PYP; 40 gm/2 g of b.wt.); 3) desferral (DFO; 12 mg/20 g of b.wt.); and 4) PYP + DFO at the respective dosages shown above. The radioactivity in each organ, blood, urine and feces was measured and referred to as percentage of the injected dose. PYP administered alone acted as a weaker chelator of iron than DFO. The combined administration of DFO and PYP contributed more than DFO or PYP separately, to the increase of urinary excretion of /sup 59/Fe and to the significant decrease of the radioiron concentration in liver (.01 less than P less than .05). The above induced changes are not, however, the additive result of the separate effect of DFO and PYP. That observation would suggest that DFO + PYP combined in a unique treatment, interact with iron through a common reaction pathway and that PYP plays in vivo a synergistic role in that interaction. The kind of iron with which DFO + PYP interacts is then suggested to be the transferrin-bound iron located in extracellular spaces of tissues.
- Research Organization:
- Biomedical Laboratory of the Isotope Department, Nuclear Research Center, Attiki, Greece
- OSTI ID:
- 6255126
- Journal Information:
- J. Pharmacol. Exp. Ther.; (United States), Vol. 224:2
- Country of Publication:
- United States
- Language:
- English
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IRON
METABOLISM
IRON 59
TRACER TECHNIQUES
TRANSFERRIN
BIOCHEMICAL REACTION KINETICS
LIVER
MICE
PYROPHOSPHATES
SYNERGISM
TISSUE DISTRIBUTION
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
ELEMENTS
EVEN-ODD NUCLEI
GLANDS
GLOBULINS
GLOBULINS-BETA
INTERMEDIATE MASS NUCLEI
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
METALLOPROTEINS
METALS
NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
TRANSITION ELEMENTS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques