Adherence of Pseudomonas aeruginosa to contact lenses

Thesis/Dissertation · Fri Jan 01 00:00:00 EST 1988

OSTI ID:6254149

Miller, M J

The purpose of this research was to examined the interactions of P. aeruginosa with hydrogel contact lenses and other substrata, and characterize adherence to lenses under various physiological and physicochemical conditions. Isolates adhered to polystyrene, glass, and hydrogel lenses. With certain lens types, radiolabeled cells showed decreased adherence with increasing water content of the lenses, however, this correlation with not found for all lenses. Adherence to rigid gas permeable lenses was markedly greater than adherence to hydrogels. Best adherence occurred near pH 7 and at a sodium chloride concentration of 50 mM. Passive adhesion of heat-killed cells to hydrogels was lower than the adherence obtained of viable cells. Adherence to hydrogels was enhanced by mucin, lactoferrin, lysozyme, IgA, bovine serum albumin, and a mixture of these macromolecules. Adherence to coated and uncoated lenses was greater with a daily-wear hydrogel when compared with an extended-wear hydrogel of similar polymer composition. Greater adherence was attributed to a higher concentration of adsorbed macromolecules on the 45% water-content lens in comparison to the 55% water-content lens.

• Research Organization: Georgia State Univ., Atlanta (USA)
• OSTI ID: 6254149
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
LENSES
PERMEABILITY
PSEUDOMONAS
ADHESION
EYES
GLASS
OPHTHALMOLOGY
POLYSTYRENE
RADIOISOTOPES
SENSE ORGANS DISEASES
TRACER TECHNIQUES
BACTERIA
BODY
BODY AREAS
DISEASES
FACE
HEAD
ISOTOPE APPLICATIONS
ISOTOPES
MATERIALS
MEDICINE
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
ORGANS
PETROCHEMICALS
PETROLEUM PRODUCTS
PLASTICS
POLYMERS
POLYOLEFINS
POLYVINYLS
SENSE ORGANS
SYNTHETIC MATERIALS
550901* - Pathology- Tracer Techniques
550600 - Medicine