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Title: Effect of chlorpromazine on rat arterial lipid synthesis, in vitro

Abstract

The effect of chlorpromazine, a major tranquilizer, on arterial lipid metabolism was studied in vitro in rat aortas incubated with (/sup 14/C)acetate and (/sup 14/C)mevalonate as lipid precursors. Chlorpromazine at a level of 0.25 mM in the incubation medium significantly reduced the incorporation of (/sup 14/C)acetate into free fatty acids (p less than 0.01) and total phospholipids (p less than 0.001) but not triglycerides. Chlorpromazine also altered the pattern of arterial phospholipids synthesized from (/sup 14/C)acetate by significantly increasing the relative proportion of phosphatidylinositol plus phosphatidylserine (p less than 0.02) and reducing the relative proportion of sphingomyelin (p less than 0.001). (/sup 14/C) Acetate incorporation into the combined fractions of steryl esters plus hydrocarbons and sterols plus diglycerides was also significantly reduced (p less than 0.001) by 0.25 mM chlorpromazine. Studies with (/sup 14/C)mevalonate showed that chlorpromazine is also an inhibitor of sterol biosynthesis in arterial tissues as evidenced by 35-40% reductions (p less than 0.05) in the formation of /sup 14/C-labeled squalene and C27 sterols.

Authors:
;
Publication Date:
Research Org.:
Diabetes and Athersclerosis Research, The Upjohn Company, Kalamazoo, MI
OSTI Identifier:
6253512
Resource Type:
Journal Article
Journal Name:
Lipids; (United States)
Additional Journal Information:
Journal Volume: 17:10
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CHLORPROMAZINE; BIOLOGICAL EFFECTS; METABOLISM; LIPIDS; BIOSYNTHESIS; AORTA; BIOCHEMICAL REACTION KINETICS; CARBON 14 COMPOUNDS; CARBOXYLIC ACIDS; IN VITRO; ISOTOPE APPLICATIONS; MOLECULAR BIOLOGY; RATS; RESEARCH PROGRAMS; TRACER TECHNIQUES; USA; AMINES; ANIMALS; ARTERIES; AZINES; BLOOD VESSELS; BODY; CARDIOVASCULAR SYSTEM; CENTRAL NERVOUS SYSTEM AGENTS; CENTRAL NERVOUS SYSTEM DEPRESSANTS; DRUGS; HETEROCYCLIC COMPOUNDS; HYPNOTICS AND SEDATIVES; KINETICS; LABELLED COMPOUNDS; MAMMALS; NORTH AMERICA; ORGANIC ACIDS; ORGANIC CHLORINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; ORGANS; PHENOTHIAZINES; PSYCHOTROPIC DRUGS; REACTION KINETICS; RODENTS; SYNTHESIS; TRANQUILIZERS; VERTEBRATES; 550501* - Metabolism- Tracer Techniques

Citation Formats

Bell, F P, and Hubert, E V. Effect of chlorpromazine on rat arterial lipid synthesis, in vitro. United States: N. p., 1982. Web. doi:10.1007/BF02534649.
Bell, F P, & Hubert, E V. Effect of chlorpromazine on rat arterial lipid synthesis, in vitro. United States. https://doi.org/10.1007/BF02534649
Bell, F P, and Hubert, E V. 1982. "Effect of chlorpromazine on rat arterial lipid synthesis, in vitro". United States. https://doi.org/10.1007/BF02534649.
@article{osti_6253512,
title = {Effect of chlorpromazine on rat arterial lipid synthesis, in vitro},
author = {Bell, F P and Hubert, E V},
abstractNote = {The effect of chlorpromazine, a major tranquilizer, on arterial lipid metabolism was studied in vitro in rat aortas incubated with (/sup 14/C)acetate and (/sup 14/C)mevalonate as lipid precursors. Chlorpromazine at a level of 0.25 mM in the incubation medium significantly reduced the incorporation of (/sup 14/C)acetate into free fatty acids (p less than 0.01) and total phospholipids (p less than 0.001) but not triglycerides. Chlorpromazine also altered the pattern of arterial phospholipids synthesized from (/sup 14/C)acetate by significantly increasing the relative proportion of phosphatidylinositol plus phosphatidylserine (p less than 0.02) and reducing the relative proportion of sphingomyelin (p less than 0.001). (/sup 14/C) Acetate incorporation into the combined fractions of steryl esters plus hydrocarbons and sterols plus diglycerides was also significantly reduced (p less than 0.001) by 0.25 mM chlorpromazine. Studies with (/sup 14/C)mevalonate showed that chlorpromazine is also an inhibitor of sterol biosynthesis in arterial tissues as evidenced by 35-40% reductions (p less than 0.05) in the formation of /sup 14/C-labeled squalene and C27 sterols.},
doi = {10.1007/BF02534649},
url = {https://www.osti.gov/biblio/6253512}, journal = {Lipids; (United States)},
number = ,
volume = 17:10,
place = {United States},
year = {Fri Oct 01 00:00:00 EDT 1982},
month = {Fri Oct 01 00:00:00 EDT 1982}
}