Measurement of DNA damage and cell killing in Chinese hamster V79 cells irradiated with aluminum characteristic ultrasoft X rays

Prise, K M; Folkard, M; Davies, S; Michael, B D

doi:10.2307/3577354

Title: Measurement of DNA damage and cell killing in Chinese hamster V79 cells irradiated with aluminum characteristic ultrasoft X rays

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Abstract

Chinese hamster V79 cells were irradiated with 1.487 keV aluminum characteristic X rays produced using a cold-cathode discharge tube. Under aerobic conditions a relative biological effectiveness (RBE) of 2.18 for cell killing in comparison to 250-kVp X rays was measured using cells grown in suspension and irradiated on membrane filters. DNA damage in the form of single-strand (ssb) and double-strand breaks (dsb) was measured using the filter elution technique. The aerobic RBEs are 1.64 for dsb induction and 0.49 for ssb induction, consistent with the view that dsb are more closely related to cell kill than ssb. A reduced oxygen enhancement ratio (OER) for cell killing was measured for Al-K X rays, but the OER for dsb induction was similar to that measured for 250-kVp X rays. A curvilinear relationship between dsb induction and dose is observed, similar to that seen for 250-kVp X rays. This agrees with the concept that ultrasoft X rays produce critical lesions similar to hard X rays but with a greater efficiency per unit dose.

Authors:: Prise, K M; Folkard, M; Davies, S; Michael, B D

Publication Date:: Wed Mar 01 00:00:00 EST 1989

Research Org.:: Mount Vernon Hospital, Northwood, Middlesex (England)

OSTI Identifier:: 6253483

Resource Type:: Journal Article

Journal Name:: Radiat. Res.; (United States)

Additional Journal Information:: Journal Volume: 117:3

Country of Publication:: United States

Language:: English

Subject:: 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ANIMAL CELLS; GENETIC RADIATION EFFECTS; STRAND BREAKS; RADIOINDUCTION; X RADIATION; RBE; AEROBIC CONDITIONS; CELL KILLING; DNA; HAMSTERS; LUNGS; OXYGEN; ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BODY; ELECTROMAGNETIC RADIATION; ELEMENTS; GENETIC EFFECTS; IONIZING RADIATIONS; MAMMALS; NONMETALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANS; RADIATION EFFECTS; RADIATIONS; RESPIRATORY SYSTEM; RODENTS; VERTEBRATES; 560120* - Radiation Effects on Biochemicals, Cells, & Tissue Culture

Citation Formats


                    Prise, K M, Folkard, M, Davies, S, and Michael, B D. Measurement of DNA damage and cell killing in Chinese hamster V79 cells irradiated with aluminum characteristic ultrasoft X rays.  United States: N. p., 1989. 
        Web.  doi:10.2307/3577354.

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                    Prise, K M, Folkard, M, Davies, S, & Michael, B D. Measurement of DNA damage and cell killing in Chinese hamster V79 cells irradiated with aluminum characteristic ultrasoft X rays.  United States.  https://doi.org/10.2307/3577354

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                    Prise, K M, Folkard, M, Davies, S, and Michael, B D. 1989.  
        "Measurement of DNA damage and cell killing in Chinese hamster V79 cells irradiated with aluminum characteristic ultrasoft X rays".  United States.  https://doi.org/10.2307/3577354.

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@article{osti_6253483,

  title        = {Measurement of DNA damage and cell killing in Chinese hamster V79 cells irradiated with aluminum characteristic ultrasoft X rays},

  author       = {Prise, K M and Folkard, M and Davies, S and Michael, B D},

  abstractNote = {Chinese hamster V79 cells were irradiated with 1.487 keV aluminum characteristic X rays produced using a cold-cathode discharge tube. Under aerobic conditions a relative biological effectiveness (RBE) of 2.18 for cell killing in comparison to 250-kVp X rays was measured using cells grown in suspension and irradiated on membrane filters. DNA damage in the form of single-strand (ssb) and double-strand breaks (dsb) was measured using the filter elution technique. The aerobic RBEs are 1.64 for dsb induction and 0.49 for ssb induction, consistent with the view that dsb are more closely related to cell kill than ssb. A reduced oxygen enhancement ratio (OER) for cell killing was measured for Al-K X rays, but the OER for dsb induction was similar to that measured for 250-kVp X rays. A curvilinear relationship between dsb induction and dose is observed, similar to that seen for 250-kVp X rays. This agrees with the concept that ultrasoft X rays produce critical lesions similar to hard X rays but with a greater efficiency per unit dose.},

  doi          = {10.2307/3577354},

  url          = {https://www.osti.gov/biblio/6253483},
  journal      = {Radiat. Res.; (United States)},
number       = ,

  volume       = 117:3,

  place        = {United States},

  year         = {Wed Mar 01 00:00:00 EST 1989},

  month        = {Wed Mar 01 00:00:00 EST 1989}

}

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