alpha. -Adrenergic vasoconstriction and receptor subtypes in large coronary arteries of calves

Young, M A; Vatner, D E; Knight, D R; Graham, R M; Homcy, C J; Vatner, S F

Title: alpha. -Adrenergic vasoconstriction and receptor subtypes in large coronary arteries of calves

Full Record
Other Related Research

Abstract

The authors investigated {alpha}-adrenoceptor subtype distribution in large coronary arteries from both functional and biochemical perspectives. The effects of intracoronary administration of the selective {alpha}{sub 1}-adrenoceptor agonist phenylephrine, of the selective {alpha}{sub 2}-adrenoceptor agonist B-HT 920 and of the mixed {alpha}{sub 1+2}-adrenoceptor agonist norepinephrine were examined on measurements of left circumflex coronary artery diameter in conscious calves. After {beta}-adrenergic blockade, equivalent reductions in large coronary artery diameter were observed with phenylephrine, B-HT, and norepinephrine. Phenylephrine-induced constrictions were abolished by prazosin, an {alpha}{sub 1}-selective antagonist, but unaffected by rauwolscine, an {alpha}{sub 2}-selective antagonist. Conversely, the B-HT-induced constriction was abolished by rauwolscine but unaffected by prazosin. Coronary constriction with norepinephrine was attenuated with either prazosin or rauwolscine and abolished by the two antagonists combined. Ligand-binding studies in which ({sup 3}H)prazosin and ({sup 3}H)rauwolscine and sarcolemmal membranes were used revealed an {alpha}{sub 1}-adrenoceptor density of 15 {plus minus} 3.1 fmol/mg protein with a dissociation constant (K{sub D}) of 0.7 {plus minus} 0.2 nM and an {alpha}{sub 2}-adrenoceptor density of 68 {plus minus} 5.1 fmol/mg protein, with a K{sub D} of 7.4 {plus minus} 1.2 nM. Thus large coronary arteries of the calf contain both {alpha}{sub 1}- and {alpha}{sub 2}-adrenoceptor subtypes, each of whichmore »« less

Authors:

Young, M A; Vatner, D E; Knight, D R; Graham, R M; Homcy, C J; Vatner, S F ^[1]

Massachusetts General Hospital, Boston (USA) New England Regional Primate Research Center, Southborough, MA (USA)

Publication Date:: Thu Dec 01 00:00:00 EST 1988

OSTI Identifier:: 6252522

Resource Type:: Journal Article

Journal Name:: American Journal of Physiology; (USA)

Additional Journal Information:: Journal Volume: 255:6; Journal ID: ISSN 0002-9513

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; LIGANDS; CROSS-LINKING; RECEPTORS; TISSUE DISTRIBUTION; VASOCONSTRICTION; PHYSIOLOGY; CALVES; CORONARIES; TRITIUM COMPOUNDS; ANIMALS; ARTERIES; BLOOD VESSELS; BODY; CARDIOVASCULAR SYSTEM; CATTLE; CHEMICAL REACTIONS; DISTRIBUTION; DOMESTIC ANIMALS; HYDROGEN COMPOUNDS; MAMMALS; MEMBRANE PROTEINS; ORGANIC COMPOUNDS; ORGANS; POLYMERIZATION; PROTEINS; RUMINANTS; VERTEBRATES; 551001* - Physiological Systems- Tracer Techniques

Citation Formats


                    Young, M A, Vatner, D E, Knight, D R, Graham, R M, Homcy, C J, and Vatner, S F. alpha. -Adrenergic vasoconstriction and receptor subtypes in large coronary arteries of calves.  United States: N. p., 1988. 
        Web.

Copy to clipboard


                    Young, M A, Vatner, D E, Knight, D R, Graham, R M, Homcy, C J, & Vatner, S F. alpha. -Adrenergic vasoconstriction and receptor subtypes in large coronary arteries of calves.  United States.

Copy to clipboard


                    Young, M A, Vatner, D E, Knight, D R, Graham, R M, Homcy, C J, and Vatner, S F. 1988.  
        "alpha. -Adrenergic vasoconstriction and receptor subtypes in large coronary arteries of calves".  United States.

Copy to clipboard


                    
@article{osti_6252522,

  title        = {alpha. -Adrenergic vasoconstriction and receptor subtypes in large coronary arteries of calves},

  author       = {Young, M A and Vatner, D E and Knight, D R and Graham, R M and Homcy, C J and Vatner, S F},

  abstractNote = {The authors investigated {alpha}-adrenoceptor subtype distribution in large coronary arteries from both functional and biochemical perspectives. The effects of intracoronary administration of the selective {alpha}{sub 1}-adrenoceptor agonist phenylephrine, of the selective {alpha}{sub 2}-adrenoceptor agonist B-HT 920 and of the mixed {alpha}{sub 1+2}-adrenoceptor agonist norepinephrine were examined on measurements of left circumflex coronary artery diameter in conscious calves. After {beta}-adrenergic blockade, equivalent reductions in large coronary artery diameter were observed with phenylephrine, B-HT, and norepinephrine. Phenylephrine-induced constrictions were abolished by prazosin, an {alpha}{sub 1}-selective antagonist, but unaffected by rauwolscine, an {alpha}{sub 2}-selective antagonist. Conversely, the B-HT-induced constriction was abolished by rauwolscine but unaffected by prazosin. Coronary constriction with norepinephrine was attenuated with either prazosin or rauwolscine and abolished by the two antagonists combined. Ligand-binding studies in which ({sup 3}H)prazosin and ({sup 3}H)rauwolscine and sarcolemmal membranes were used revealed an {alpha}{sub 1}-adrenoceptor density of 15 {plus minus} 3.1 fmol/mg protein with a dissociation constant (K{sub D}) of 0.7 {plus minus} 0.2 nM and an {alpha}{sub 2}-adrenoceptor density of 68 {plus minus} 5.1 fmol/mg protein, with a K{sub D} of 7.4 {plus minus} 1.2 nM. Thus large coronary arteries of the calf contain both {alpha}{sub 1}- and {alpha}{sub 2}-adrenoceptor subtypes, each of which elicits constriction of the large coronary artery in the conscious animal.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/6252522},
  journal      = {American Journal of Physiology; (USA)},

  issn         = {0002-9513},

  number       = ,

  volume       = 255:6,

  place        = {United States},

  year         = {Thu Dec 01 00:00:00 EST 1988},

  month        = {Thu Dec 01 00:00:00 EST 1988}

}

Copy to clipboard

Journal Article:

Other availability

Find in Google Scholar

Search WorldCat to find libraries that may hold this journal

Save / Share:

Export Metadata

Save to My Library

Similar records in OSTI.GOV collections:

Postsynaptic alpha-adrenoceptors, calcium mobilization and (/sup 3/H), 4-dihydropyridine binding in vascular smooth muscle of rat tail artery

Thesis/Dissertation Su, C

Pharmacologic characterization of post-synaptic ..cap alpha..-adrenoceptors in rat tail artery was examined by using selective agonists and antagonists. In this tissue, the ..cap alpha..-adrenoceptor agonists employed all produced concentration-dependent mechanical responses with rank order of potency, clonidine > norepinephrine > norepinephrine > phenylephrine > UK > 14304 > B-HT 920. This order of agonists activities not consistent with a simple classification into ..cap alpha../sub 1/- and ..cap alpha../sub 2/-adrenoceptors in the rat tail artery. Antagonism by prazosin and yohimbine of phenylephrine, norepinephrine and clonidine responses did not reveal the anticipated discrimination between ..cap alpha../sub 1/- and ..cap alpha../sub 2/-adrenoceptors. Potassiummore »« less
Characteristics of adrenoceptors and (/sup 3/H)nitrendipine receptors of porcine vascular smooth muscle: differences between coronary artery and aorta

Journal Article Nishimura, J; Kanaide, H; Nakamura, M - Circ. Res.; (United States)

Characteristics of the bindings of (/sup 3/H)(-)dihydroalprenolol, (/sup 125/I)(-)iodocyanopindolol, (/sup 3/H)prazosin, (/sup 3/H)yohimbine, and (/sup 3/H)nitrendipine to porcine coronary membranes were investigated and the results compared with studies of porcine aortic membranes. In the equilibrium binding study carried out in sarcolemma-enriched fractions, there were no major differences in the Kd values of these radioligands between coronary artery and aorta. However, the densities of beta-, alpha 1-, and alpha 2-adrenoceptors and (/sup 3/H)nitrendipine receptors of coronary artery were 258, 12, 12, and 561 fmol/mg protein, respectively, while those of aorta were 37, 525, 1000, and 215 fmol/mg protein. beta-Adrenergic agonists competedmore »« less
https://doi.org/10.1161/01.RES.60.6.837
Identification and characterization of (/sup 3/H)-rauwolscine binding to alpha2-adrenoceptors in the canine saphenous vein

Journal Article Gout, B - Life Sci.; (United States)

The biochemical exploration of the alpha2-adrenergic receptors was investigated in the canine saphenous vein using the highly selective alpha2-adrenergic antagonist rauwolscine as a tritiated ligand. Following an enzymatic digestive pretreatment, the authors isolated a purified smooth muscle cell membranes fraction from saphenous veins in quantity sufficient to permit them to study the venous alpha2-adrenoreceptor content. The binding of tritiated rauwolscine was rapid, specific, saturable and reversible. The presence of high affinity sites with a density of binding Bmax of 125.2 /+ -/ 43.1 fmol/mg protein was demonstrated on a unique class of non interacting sites. The kinetically derived Kd wasmore »« less
https://doi.org/10.1016/0024-3205(88)90569-3
Different affinity states of alpha-1 adrenergic receptors defined by agonists and antagonists in bovine aorta plasma membranes

Journal Article Jagadeesh, G; Deth, R - J. Pharmacol. Exp. Ther.; (United States)

Evidence for a nonlinear relationship between alpha-1 adrenergic receptor occupancy and tissue responses, together with the finding of different affinity states for agonist binding, has raised the possibility of functional heterogeneity of alpha-1 adrenergic receptors. We have conducted studies to examine: 1) binding characteristics of (/sup 3/H)prazosin, 2) competition of antagonists at these sites and 3) different affinity states of the receptor for agonists and modulation of these states by 5'-guanylylimidodiphosphate (Gpp(NH)p). A plasma membrane-enriched vesicular fraction (F2; 15%/33% sucrose interphase) was prepared from the muscular medial layer of bovine thoracic aorta. (/sup 3/H)Prazosin binding was characterized by a monophasicmore »« less
Synergistic alpha-1 and alpha-2 adrenergic stimulation of rat proximal nephron Na+/H+ exchange

Journal Article Gesek, F; Cragoe, Jr, E; Strandhoy, J - J. Pharmacol. Exp. Ther.; (United States)

Both alpha-1 and alpha-2 adrenoceptors have been localized to the renal cortex, with the majority of binding sites on the proximal tubule. Because the major regulator of Na+ uptake into the proximal tubule is the Na+/H+ exchanger, and because alpha-1 and alpha-2 adrenoceptors stimulate it in other tissues, we tested the hypothesis that both alpha adrenoceptor subtypes can increase Na+ uptake into the proximal nephron by stimulating the Na+/H+ antiporter. Enhancement of Na+ transport by agonists was studied in isolated rat proximal tubules by determining the uptake of 22Na that was suppressible by the Na+/H+ inhibitor, 5-(N-ethyl-N-isopropyl)amiloride (EIPA). The phorbolmore »« less

Similar Records