Study of protein modifications induced by phorbol ester tumor promoters in mouse skin
The purpose of this study was to determine if the phorbol ester tumor promoter 12-0-tetradecanoylphorbol-13-acetate (TPA) induced any specific changes in mouse epidermal proteins using the high resolution technique of two-dimensional electrophoresis. To accomplish this goal of determining the specificity and possibly the stage in promotion with which these protein changes were associated, epidermal proteins were analyzed (1) after treatment of adult mouse epidermis with several weakly promoting hyperplasiogenic agents, (2) following treatment with TPA in combination with various inhibitors of tumor promotion, (3) in basal kerotinocytes isolated from adult epidermis following treatment with TPA or several weakly promoting agents, and (4) during an initiation-promotion experiment. Evidence was found which indicated that the potent tumor promoter TPA as well as the weakly promoting hyperplasiogenic agents, mezerein, ethylphenylpropiolate (EPP), and mechanical abrasion, induced similar modifications of epidermal proteins, particularly among the keratins. These keratin modifications progressed with time following treatment resulting in a keratin pattern which resembled that of newborn epidermis.
- Research Organization:
- Oak Ridge Associated Universities, Inc., TN (USA); Tennessee Univ., Knoxville (USA)
- DOE Contract Number:
- AC05-76OR00033
- OSTI ID:
- 6251390
- Report Number(s):
- DOE/TIC-11494; ON: DE81031985
- Resource Relation:
- Other Information: Thesis. Submitted to the Univ. of Tennessee, Knoxville, TN
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
EPIDERMIS
PROTEINS
TUMOR PROMOTERS
BIOLOGICAL EFFECTS
CARCINOGENESIS
ELECTROPHORESIS
EXPERIMENTAL DATA
MICE
PHORBOL ESTERS
ANIMAL TISSUES
ANIMALS
BODY
CARCINOGENS
DATA
EPITHELIUM
ESTERS
INFORMATION
MAMMALS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PROMOTERS
RODENTS
SKIN
TISSUES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)