Detergent (sodium dodecyl sulfate) shock proteins in Escherichia coli
Journal Article
·
· Journal of Bacteriology; (USA)
OSTI ID:6249539
- Univ. of Nebraska, Lincoln (USA)
The protein composition of Escherichia coli W3110 grown in the presence and absence of 5% sodium dodecyl sulfate (SDS) was examined by two-dimensional gel electrophoresis. In SDS-grown cells, at least 4 proteins were turned on, 13 were turned off, 15 were elevated, and 15 were depressed. The 19 unique and elevated SDS-induced spots constituted 7.91% of the total 35S-labeled protein. There was no apparent overlap between these 19 detergent (SDS) stress proteins and those of other known bacterial stress responses. The detergent stress stimulon is a distinct and independent stimulon. Its physiological relevance probably derives from the presence of bile salts in animal gastrointestinal tracts.
- OSTI ID:
- 6249539
- Journal Information:
- Journal of Bacteriology; (USA), Vol. 173:1; ISSN 0021-9193
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ESCHERICHIA COLI
BIOLOGICAL STRESS
PROTEINS
BIOSYNTHESIS
METHIONINE
SULFUR 35
TRACER TECHNIQUES
TWO-DIMENSIONAL ELECTROPHORESIS
AMINO ACIDS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MICROORGANISMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIOISOTOPES
SULFUR ISOTOPES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques
550701 - Microbiology- Tracer Techniques
ESCHERICHIA COLI
BIOLOGICAL STRESS
PROTEINS
BIOSYNTHESIS
METHIONINE
SULFUR 35
TRACER TECHNIQUES
TWO-DIMENSIONAL ELECTROPHORESIS
AMINO ACIDS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MICROORGANISMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIOISOTOPES
SULFUR ISOTOPES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques
550701 - Microbiology- Tracer Techniques