Catalyzed enzyme electrodes

Patent · Tue Jul 13 00:00:00 EDT 1993

OSTI ID:6247863

Zawodzinski, T A; Wilson, M S; Rishpon, J; Gottesfeld, S

An enzyme electrode is prepared with a composite coating on an electrical conductor. The composite coating is formed from a casting solution of a perfluorosulfonic acid polymer, an enzyme, and a carbon supported catalyst. The solution may be cast directly on the conductor surface or may be formed as a membrane and applied to the surface. The perfluorosulfonic acid ionomer formed from the casting solution provides an insoluble biocompatible protective matrix for the enzyme and acts to retain the enzyme for long term availability in the electrode structure. The carbon supported catalyst provides catalytic sites throughout the layer for the oxidation of hydrogen peroxide from the enzyme reactions. The carbon support then provides a conductive path for establishing an electrical signal to the electrical conductor. In one embodiment, the electrical conductor is a carbon cloth that permits oxygen or other gas to be introduced to the perfluorosulfonic polymer to promote the enzyme reaction independent of oxygen in the solution being tested.

• Assignee: Dept. of Energy, Washington, DC (United States)
• Patent Number(s): US 5227042; A
• Application Number: PPN: US 7-883746
• OSTI ID: 6247863
• Resource Relation: Patent File Date: 15 May 1992
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
IMMOBILIZED ENZYMES
CHEMICAL PREPARATION
CARBON
CATALYST SUPPORTS
ELECTRIC CONDUCTORS
ELECTRODES
ENZYME ACTIVITY
ENZYMES
HYDROGEN PEROXIDE
OXIDATION
POLYMERS
SULFONIC ACIDS
SURFACE COATING
CHEMICAL REACTIONS
DEPOSITION
ELEMENTS
HYDROGEN COMPOUNDS
NONMETALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PEROXIDES
PROTEINS
SYNTHESIS
550200* - Biochemistry