Influence of dehydroepiandrosterone on G-6-PD activity and /sup 3/H-thymidine uptake of human lymphocytes in vitro

Journal Article · Thu Jan 01 00:00:00 EST 1987 · Toxicol. Pathol.; (United States)

DOI:https://doi.org/10.1177/019262338701500220· OSTI ID:6245875

Ennas, M G; Laconi, S; Dessi, S; Milia, G; Murru, M R; Manconi, P E

Dehydroepiandrosterone (DHEA) was found to inhibit experimental cancer development in mouse and rat lung, colon and mammary gland. Since DHEA is a potent inhibitor of mammalian G-6-PD, the hypothesis that the compound could inhibit cell proliferation through an inhibition of the pentose phosphate pathway has been formulated. We studied the effects of DHEA on the proliferation in vitro of human lymphocytes induced by several mitogens (PHA, ConA and PWM), measuring /sup 3/H-thymidine uptake. DHEA inhibited /sup 3/H-thymidine uptake of mitogen-stimulated cells from both G-6-PD+ and G-6-PD- (mediterranean type deficiency) individuals in a dose-dependent and reversible fashion. The inhibitory effect was found even if DHEA was added to cells in the last hours of culture, simultaneously with the addition of /sup 3/H-thymidine. These data suggest that the inhibition of thymidine uptake induced by DHEA on human lymphocytes probably does not depend on the inhibition of G-6-PD.

• Research Organization: Universita di Cagliari, Italy
• OSTI ID: 6245875
• Journal Information: Toxicol. Pathol.; (United States), Vol. 15:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANDROSTERONE
BIOLOGICAL EFFECTS
LYMPHOCYTES
CELL PROLIFERATION
OXIDOREDUCTASES
ENZYME ACTIVITY
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
IN VITRO
INHIBITION
MAN
MITOGENS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANDROGENS
ANDROSTANES
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
ENZYMES
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PYRIMIDINES
RIBOSIDES
SOMATIC CELLS
STEROID HORMONES
STEROIDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques