Sequence and transcription analysis of the human cytomegalovirus DNA polymerase gene

Journal Article · Thu Jan 01 00:00:00 EST 1987 · J. Virol.; (United States)

OSTI ID:6244419

Kouzarides, T; Bankier, A T; Satchwell, S C; Weston, K; Tomlinson, P; Barrell, B G

DNA sequence analysis has revealed that the gene coding for the human cytomegalovirus (HCMV) DNA polymerase is present within the long unique region of the virus genome. Identification is based on extensive amino acid homology between the predicted HCMV open reading frame HFLF2 and the DNA polymerase of herpes simplex virus type 1. The authors present here a 5280 base-pair DNA sequence containing the HCMV pol gene, along with the analysis of transcripts encoded within this region. Since HCMV pol also shows homology to the predicted Epstein-Barr virus pol, they were able to analyze the extent of homology between the DNA polymerases of three distantly related herpes viruses, HCMV, Epstein-Barr virus, and herpes simplex virus. The comparison shows that these DNA polymerases exhibit considerable amino acid homology and highlights a number of highly conserved regions; two such regions show homology to sequences within the adenovirus type 2 DNA polymerase. The HCMV pol gene is flanked by open reading frames with homology to those of other herpes viruses; upstream, there is a reading frame homologous to the glycoprotein B gene of herpes simplex virus type I and Epstein-Barr virus, and downstream there is a reading frame homologous to BFLF2 of Epstein-Barr virus.

• Research Organization: Medical Research Council, Cambridge (England)
• OSTI ID: 6244419
• Journal Information: J. Virol.; (United States), Vol. 61:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA
AUTORADIOGRAPHY
DNA POLYMERASES
DNA SEQUENCING
VIRUSES
TRANSCRIPTION
GENES
HERPES SIMPLEX
PHOSPHORUS 32
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
ENZYMES
INFECTIOUS DISEASES
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIOISOTOPES
SKIN DISEASES
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VIRAL DISEASES
550201* - Biochemistry- Tracer Techniques