Sequence of the NH/sub 2/-terminal 204-residue fragment of the heavy chain of rabbit skeletal muscle myosin
The amino acid sequence of the M/sub r/ = 23,000 peptide that is generated by limited tryptic digestion of rabbit skeletal muscle heavy meromyosin was detrmined. This fragment represents the NH/sub 2/-terminal 204 residues of the heavy chain of myosin, and its sequence is Ac-S-S-D-A-D-M-A-V/I-F-G-E-A-A-P-Y-L-R-K-S-20-E-K-E-R-I-E-A-Q-N-K-P-F-D-A-K/MML-N-S-V-F-40-V-A/V-D-P-K-E-S-Y/I-V-K-A/E-T-V-Q-S-R-E-G-G-60-K-V-T-V/A-K-T-E-A-G-A-S-V-T-V-K-E-D-Q-V-F-80-P-M-N-P-P-K-Y-D-K-I-E-D-M-A-M-M-T-H-L-H-E-100-P-A-V-L-Y--N-L-K-E-R-Y-A-A-W-M-I-Y-T-Y-S-120-G-L-F-C-V-T-V-N-P-Y-TML-W-L-P-V-Y-N-A-E-V-140-V-T-A-Y-R-G-K-K-R-Q-E-A-P-P-H-I-F-S-I-S-160-D-N-A-Y-Q-F-M-L-T-D-R-E-N-Q-S-I-L-I-T-G-E-180-S-G-A-G-K-T-V-N-T-K-R-V-I-Q-Y-F-A-T-I-A-200-I/V-T-G-D-K. This peptide contains the lysine residue (Lys 83) whose reaction with 2,4,6-trinitrobenzenesulfonate alters the enzymatic activity of mysoin, as well as two types of methylated lysines. Position 34 is occupied by element of-N-monomethyllysine and lysine in an approximate 60:40 ratio, while position 129 is fully occupied by one of the two element of-N-trimethyllysines in myosin heavy chain. There is evidence suggesting that this fragment contains residues that contribute to the ATP-binding site of myosin; the sequence surrounding the element of-N-trimethyllysine is devoid of charges and could form a hydrophobic pocket for binding of the adenine moiety while the element of-N-trimetyllysine, which carries a positive charge at all pH values, could bind an ATP phosphate group.
- Research Organization:
- Brookhaven National Lab., Upton, NY
- OSTI ID:
- 6241857
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 258:21
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MUSCLES
PEPTIDES
MYOSIN
AMINO ACID SEQUENCE
ADENINES
ATP
CHEMICAL BONDS
ENZYME ACTIVITY
LYSINE
MOLECULAR BIOLOGY
RABBITS
AMINES
AMINO ACIDS
ANIMALS
ANTIMETABOLITES
AROMATICS
AZAARENES
CARBOXYLIC ACIDS
DRUGS
GLOBULINS
HETEROCYCLIC COMPOUNDS
MAMMALS
MOLECULAR STRUCTURE
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PURINES
VERTEBRATES
550200* - Biochemistry