Mutagenicity in Salmonella of hazardous wastes and urine from rats fed these wastes

Technical Report · Mon Jun 01 00:00:00 EDT 1987

OSTI ID:6240444

DeMarini, D M; Inmon, J P; Simmons, J E; Berman, E; Pasley, T C

Fifteen hazardous industrial waste samples were evaluated for mutagenicity in the Salmonella plate-incorporation assay using strains TA98 and TA100 in the presence and absence of Aroclor 1254-induced rat liver S9. Dichloromethane/methanol extracts of the crude wastes also were evalauted. Seven of the crude wastes were mutagenic, but only 2 of the extract of these 7 wastes were mutagenic; extracts of 2 additional wastes also were mutagenic. In addition, 10 of the crude wastes were administered by gavage to F-344 rats, and 24-H urine samples were collected. Of the 10 raw urines evaluated, 3 were mutagenic in strain TA98 in the presence of S9 and B-glucuronidase. To the authors' knowledge, this is the first report of the mutagenicity of urine from rodents exposed to hazardous wastes. Based on the present results, the use of only strain TA98 in the presence of S9 might be adequate for general screening of hazardous wastes or waste extracts for genotoxicity.

• Research Organization: Environmental Protection Agency, Research Triangle Park, NC (USA). Health Effects Research Lab.
• OSTI ID: 6240444
• Report Number(s): PB-87-203394/XAB; EPA-600/D-87/208
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CARCINOGENS
UPTAKE
HAZARDOUS MATERIALS
MUTAGENS
INDUSTRIAL WASTES
SALMONELLA
BIOASSAY
RATS
TOXICITY
URINE
ANIMALS
BACTERIA
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
MAMMALS
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MICROORGANISMS
RODENTS
VERTEBRATES
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560300* - Chemicals Metabolism & Toxicology