New techniques for growing anaerobic bacteria: experiments with Clostridium butyricum and Clostridium acetobutylicum

Journal Article · Sat Jan 01 00:00:00 EST 1983 · Biotechnol. Bioeng. Symp.; (United States)

OSTI ID:6238979

Adler, H I; Crow, W D; Hadden, C T; Hall, J; Machanoff, R

Stable membrane fragments derived from Escherichia coli produce and maintain strict anaerobic conditions when added to liquid or solid bacteriological media. Techniques for growing Clostridium butyricum and Clostridium acetobutylicum in membrane-containing media are described. Liquid cultures initiated by very small inocula can be grown in direct contact with air. In solid media, colonies develop rapidly from individual cells even without incubation in anaerobic jars or similar devices. Observations on growth rates, spontaneous mutations, radiation, and oxygen sensitivity of anaerobic bacteria have been made using these new techniques.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26; AS05-76EV04568
• OSTI ID: 6238979
• Journal Information: Biotechnol. Bioeng. Symp.; (United States), Vol. 13
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CLOSTRIDIUM ACETOBUTYLICUM
CULTIVATION TECHNIQUES
CLOSTRIDIUM BUTYRICUM
COLONY FORMATION
BIOLOGICAL RADIATION EFFECTS
ULTRAVIOLET RADIATION
BIOLOGICAL EFFECTS
ANAEROBIC CONDITIONS
CELL MEMBRANES
CULTURE MEDIA
ESCHERICHIA COLI
GROWTH
IONIZING RADIATIONS
MUTATIONS
OXYGEN
REMOVAL
SENSITIVITY
BACTERIA
CELL CONSTITUENTS
CLOSTRIDIUM
ELECTROMAGNETIC RADIATION
ELEMENTS
MEMBRANES
METHANOGENIC BACTERIA
MICROORGANISMS
NONMETALS
RADIATION EFFECTS
RADIATIONS
550700* - Microbiology
560131 - Radiation Effects on Microorganisms- Basic Studies- (-1987)