Structural characterization of proteoglycans produced by testicular peritubular cells and Sertoli cells

Journal Article · Wed Sep 25 00:00:00 EDT 1985 · J. Biol. Chem.; (United States)

OSTI ID:6235529

Skinner, M K; Fritz, I B

The structural characteristics of proteoglycans produced by seminiferous peritubular cells and by Sertoli cells are defined. Peritubular cells secrete two proteoglycans designated PC I and PC II. PC I is a high molecular mass protein containing chondroitin glycosaminoglycan (GAG) chains (maximum 70 kDa). PC II has a protein core of 45 kDa and also contains chondroitin GAG chains (maximum 70 kDa). Preliminary results imply that PC II may be a degraded or processed form of PC I. Sertoli cells secrete two different proteoglycans, designated SC I and SC II. SC I is a large protein containing both chondroitin (maximum 62 kDa) and heparin (maximum 15 kDa) GAG chains. Results obtained suggest that this novel proteoglycan contains both chondroitin and heparin GAG chains bound to the same core protein. SC II has a 50-kDa protein core and contains chondroitin (maximum 25 kDa) GAG chains. A proteoglycan obtained from extracts of Sertoli cells is described which contains heparin (maximum 48 kDa) GAG chains. In addition, Sertoli cells secrete a sulfoprotein, SC III, which is not a proteoglycan. The stimulation by follicle-stimulating hormone of the incorporation of (TVS)SO2) U) into moieties secreted by Sertoli cells is shown to represent an increased production or sulfation of SC III, and not an increased production or sulfation of proteoglycans. Results are discussed in relation to the possible functions of proteoglycans in the seminiferous tubule.

• Research Organization: Univ. of Toronto, Ontario
• OSTI ID: 6235529
• Journal Information: J. Biol. Chem.; (United States), Vol. 21
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOPROTEINS
BIOCHEMISTRY
MOLECULAR STRUCTURE
SULFUR 35
TRACER TECHNIQUES
ANIMAL CELLS
FSH
MALE GENITALS
RATS
SULFATES
TESTES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CHEMISTRY
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
GONADOTROPINS
GONADS
HORMONES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
RADIOISOTOPES
RODENTS
SACCHARIDES
SULFUR COMPOUNDS
SULFUR ISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques