Resistance of platelet proteins to effects of ionizing radiation

Journal Article · Sat Dec 01 00:00:00 EST 1990 · Journal of Laboratory and Clinical Medicine; (USA)

OSTI ID:6227165

Prodouz, K N; Habraken, J W; Moroff, G ^[1]

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1. Center for Biologics Evaluation and Research, Bethesda, MD (USA)

Gamma irradiation of blood components prevents lymphocyte-induced graft-versus-host disease after transfusion in immunocompromised individuals. In this report we demonstrate the resistance of blood platelet proteins to gamma radiation-induced protein cleavage and aggregate formation when platelet concentrates were treated with a dose of 5000 rad. Results of one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis of total platelet protein and cytoskeletal protein preparations indicate that platelet proteins are neither cleaved nor cross-linked under these conditions of irradiation. These results support those of a previous study that documented the lack of any adverse effect of 5000 rad gamma radiation on in vitro platelet properties.

• OSTI ID: 6227165
• Journal Information: Journal of Laboratory and Clinical Medicine; (USA), Vol. 116:6; ISSN 0022-2143
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ERYTHROCYTES
BIOLOGICAL RADIATION EFFECTS
PROTEINS
RADIOLYSIS
GAMMA RADIATION
GRAFT-HOST REACTION
MAN
RADIATION DOSES
TRANSFUSIONS
TWO-DIMENSIONAL ELECTROPHORESIS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
DOSES
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
IONIZING RADIATIONS
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
PRIMATES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
THERAPY
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture