U.S. Department of EnergyOffice of Scientific and Technical Information
Molecular biological enhancement of coal biodesulfurization
Abstract
The following work was accomplished during the Sixth Quarterly Report period in the investigation of the microbial-mediated release of sulfur from coal as viable industrial process for coal desulfurization: Analysis of the carbon source utilization profile by strain C-18 using the Microlog 2N system indicated that this strain was not among the more than 400 bacteria included in this data base. A gradient elution HPLC system developed for separating 4S metabolites gave good resolution of 4S standards except for o,o'-biphenol and o-phenylphenol. The pC18L cosmid library purified by cesium chloride-ethidium bromide density gradient equilibrium centrifugation could be inserted by electroporation into Escherichia coli HB101 to give clones on the order of 10{sup 6} transformants/{mu}g DNA. However, no clones were obtained after electroporation of this preparation with Pseudomonas putida ATCC 33015. Electroporation of the HB101-packaged pC18L (cesium banded) cosmid library into P. putida ATCC 33015 resulted in very low transformation rates. Apparently, the presence of the Tol plasmid in the 33015 strain interfered with this electrotransformation. Battelle bacterial isolates obtained from fuel-contaminated soil showed evidence for utilizing a variety of organosulfonate and organosulfone compounds as sole sulfur sources. Further confirmatory experiments are planned. Antibiotic and metal sensitivity and resistance evaluation ofmore »
- Authors:
-
- Battelle, Columbus, OH (USA)
- Ohio State Univ., Columbus, OH (USA)
- Publication Date:
- Research Org.:
- Battelle, Columbus, OH (USA)
- Sponsoring Org.:
- DOE/FE
- OSTI Identifier:
- 6222547
- Report Number(s):
- DOE/PC/89902-T3
ON: DE91007435
- DOE Contract Number:
- AC22-89PC89902
- Resource Type:
- Technical Report
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 01 COAL, LIGNITE, AND PEAT; 59 BASIC BIOLOGICAL SCIENCES; COAL; DESULFURIZATION; ESCHERICHIA COLI; GENES; DNA-CLONING; THIOBACILLUS FERROXIDANS; ANTIBIOTICS; BIODEGRADATION; METABOLITES; ORGANIC SULFUR COMPOUNDS; PLASMIDS; PROGRESS REPORT; PSEUDOMONAS; SULFUR CONTENT; ANTI-INFECTIVE AGENTS; BACILLUS; BACTERIA; CARBONACEOUS MATERIALS; CELL CONSTITUENTS; CHEMICAL REACTIONS; CLONING; DECOMPOSITION; DNA HYBRIDIZATION; DOCUMENT TYPES; DRUGS; ENERGY SOURCES; FOSSIL FUELS; FUELS; HYBRIDIZATION; MATERIALS; MICROORGANISMS; ORGANIC COMPOUNDS; SULFUR-OXIDIZING BACTERIA; 010402* - Coal, Lignite, & Peat- Purification & Upgrading; 550700 - Microbiology; 550400 - Genetics
Citation Formats
Litchfield, J H, Fry, I, Wyza, R E, Palmer, D T, Zupancic, T J, Conkle, H N, Delgado, O, and Tuovinen, O H. Molecular biological enhancement of coal biodesulfurization. United States: N. p., 1990.
Web.
Litchfield, J H, Fry, I, Wyza, R E, Palmer, D T, Zupancic, T J, Conkle, H N, Delgado, O, & Tuovinen, O H. Molecular biological enhancement of coal biodesulfurization. United States.
Litchfield, J H, Fry, I, Wyza, R E, Palmer, D T, Zupancic, T J, Conkle, H N, Delgado, O, and Tuovinen, O H. 1990.
"Molecular biological enhancement of coal biodesulfurization". United States.
@article{osti_6222547,
title = {Molecular biological enhancement of coal biodesulfurization},
author = {Litchfield, J H and Fry, I and Wyza, R E and Palmer, D T and Zupancic, T J and Conkle, H N and Delgado, O and Tuovinen, O H},
abstractNote = {The following work was accomplished during the Sixth Quarterly Report period in the investigation of the microbial-mediated release of sulfur from coal as viable industrial process for coal desulfurization: Analysis of the carbon source utilization profile by strain C-18 using the Microlog 2N system indicated that this strain was not among the more than 400 bacteria included in this data base. A gradient elution HPLC system developed for separating 4S metabolites gave good resolution of 4S standards except for o,o'-biphenol and o-phenylphenol. The pC18L cosmid library purified by cesium chloride-ethidium bromide density gradient equilibrium centrifugation could be inserted by electroporation into Escherichia coli HB101 to give clones on the order of 10{sup 6} transformants/{mu}g DNA. However, no clones were obtained after electroporation of this preparation with Pseudomonas putida ATCC 33015. Electroporation of the HB101-packaged pC18L (cesium banded) cosmid library into P. putida ATCC 33015 resulted in very low transformation rates. Apparently, the presence of the Tol plasmid in the 33015 strain interfered with this electrotransformation. Battelle bacterial isolates obtained from fuel-contaminated soil showed evidence for utilizing a variety of organosulfonate and organosulfone compounds as sole sulfur sources. Further confirmatory experiments are planned. Antibiotic and metal sensitivity and resistance evaluation of T. ferrooxidans strains have led to the identification of one mercury-resistent strain (DSM 5083) and one selenite-sensitive strain (TFI 85). Plasmid and chromosomal DNA have been prepared from T. ferrooxidans strains. One plasmid, pTFI 91, is being mapped. 4 refs., 1 fig., 4 tabs.},
doi = {},
url = {https://www.osti.gov/biblio/6222547},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Dec 14 00:00:00 EST 1990},
month = {Fri Dec 14 00:00:00 EST 1990}
}