Use of sputter-deposited 316L stainless steel ultrathin films for microbial influenced corrosion studies
- Montana State Univ., Bozeman, MT (United States)
- Univ. of Tennessee, Knoxville, TN (United States)
Ultra thin films (12nm) were sputter deposited onto cylindrical germanium internal reflection elements pre-coated with a thin (2 nm) layer of Cr{sub 2}O{sub 3}. Two crystals were inserted into Circle cell flow-through chambers and mounted on the optical bench of an Fourier Transform Infrared (FT-IR) spectrometer. One chamber was maintained as a sterile control while the other was sequentially inoculated with four bacterial species: Psudomonas aeruginosa, Bacillus subtillis, Hafnia alvei, and Desulfovibrio gigas, in that order. The water absorption band (1640cm{sup -4}) was monitored and used to follow that deterioration of the ultra thin films. In this respect, the sterile control and inoculated films exhibited only slight differences during the 1000h course of the experiment. Assay of the visible biofilm that has accumulated on the surface of the inoculated crystal after 1000h revealed that the film incorporated viable cells from all four strains.
- OSTI ID:
- 62223
- Report Number(s):
- CONF-921101-Vol.294; CNN: Grant DMR9196070; TRN: 95:002922-0039
- Resource Relation:
- Conference: 16. Materials Research Society (MRS) fall meeting, Boston, MA (United States), 30 Nov - 5 Dec 1992; Other Information: PBD: 1993; Related Information: Is Part Of Scientific basis for nuclear waste management XVI; Interrante, C.G. [ed.] [Nuclear Regulatory Commission, Washington, DC (United States)]; Pabalan, R.T. [ed.] [Southwest Research Inst., San Antonio, TX (United States)]; PB: 959 p.
- Country of Publication:
- United States
- Language:
- English
Similar Records
Formation of Pseudomonas aeruginosa Biofilms in Full-thickness Scald Burn Wounds in Rats
Quantitative SIMS Imaging of Agar-Based Microbial Communities