Preparation of plasmid DNA by gamma-irradiation of recA cells
If recA bacteria are exposed to appropriate doses of gamma-irradiation, nondividing cells which can sustain the multiplication of ''small'' plasmids are produced. The gamma-irradiation technique has a number of advantages over other methods for preparing pure plasmid DNA: (1) there is little, if any, contamination of DNA preparations by chromosomal DNA owing to extensive degradation of the irradiated DNA by endogenous nucleases, (2) there is no need to introduce a uvr mutation to the host bacteria (there is when UV is used to inactivate the chromosome), (3) the method is extremely simple to work with since operations are not limited by considerations of volume and cell density, and (4) there is no need to transfer material from container to container. Yields of plasmid DNA obtained by the gamma-irradiation technique compare favorably with those obtained by other methods.
- Research Organization:
- La Trobe Univ., Bundoora, Victoria, Australia
- OSTI ID:
- 6221292
- Journal Information:
- Plasmid; (United States), Vol. 10:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
BIOLOGICAL RADIATION EFFECTS
DNA
SAMPLE PREPARATION
PLASMIDS
BACTERIA
BIOLOGICAL EFFECTS
DOSE-RESPONSE RELATIONSHIPS
GAMMA RADIATION
CELL CONSTITUENTS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
560130* - Radiation Effects on Microorganisms