Measuring chlorophyll. cap alpha. and /sup 14/C-labeled photosynthate in aquatic angiosperms by the use of a tissue solubilizer. [/sup 14/C-labelled photosynthate]

Journal Article · Fri Jan 01 00:00:00 EST 1982 · Plant Physiol.; (United States)

OSTI ID:6219362

Beer, S; Stewart, A J; Wetzel, R G

A compound that quantitatively correlated with chlorophyll ..cap alpha.. could be measured fluorometrically in the extracts of leaves of three aquatic angiosperms (Myriophyllum heterophyllum Michx., Potamogeton crispus L., Elodea canadensis Michx.) treated with the tissue solubilizer BTS-450. Fluorescent characteristics of the solubilized plant tissues were stable for several weeks in the dark at temperatures up to 60/sup 0/C but rapidly degraded in sunlight or when acidified. /sup 14/C-Labeled photosynthate, which had been fixed by leaf discs during 1- to 10-hour exposure to H/sup 14/CO/sub 3/, was also readily extracted by the tissue solubilizer. Solubilizer extraction can, therefore, be used to determine both chlorophyll ..cap alpha.. content and /sup 14/C incorporation rates in the same leaf sample. The method is practical, because no grinding is required, the fluorescent characteristics of the extracts are stable, and analyses can be performed with very little plant material (about 3 milligrams).

• Research Organization: Michigan State Univ., Hickory Corners
• DOE Contract Number: AC02-76EV01599
• OSTI ID: 6219362
• Journal Information: Plant Physiol.; (United States), Vol. 69
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHLOROPHYLL
EXTRACTION
MEASURING METHODS
ABSORPTION SPECTRA
AQUATIC ORGANISMS
CARBON 14 COMPOUNDS
EXPERIMENTAL DATA
FLUORESCENCE
LABELLED COMPOUNDS
PLANTS
RADIOASSAY
CARBOXYLIC ACIDS
DATA
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INFORMATION
LUMINESCENCE
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOCHROMES
PIGMENTS
PORPHYRINS
SEPARATION PROCESSES
SPECTRA
550201* - Biochemistry- Tracer Techniques