Adenovirus E1A gene autorepression: revertants of an E1A promoter mutation encode altered E1A proteins

Journal Article · Tue Dec 01 00:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.23.8185· OSTI ID:6218582

Larsen, P L; Tibbetts, C

Revertants have been isolated from Ad3hr15, a mutant of human adenovirus type 3 that carries a defective E1A promoter. Transcription of these revertant E1A genes is restored - from nil for Ad3hr15 mutant to levels exceeding that of the wild-type virus. The mutant Ad3hr15 virus and the revertants all have an aberrant E1A promoter that contains two short tandem duplications of viral DNA sequence. The E1A gene-coding region of the mutant is the same as that for wild-type adenovirus type 3, whereas the revertants are characterized by short in-frame deletions within the 5' exon region of their E1A genes. Location of these reverting, second-site deletions is discussed in relation to E1A gene autoregulation and the evolved diversity of E1A-related oncogenic potential among different human adenoviruses.

• Research Organization: Vanderbilt Univ. School of Medicine, Nashville, TN (USA)
• OSTI ID: 6218582
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:23
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ADENOVIRUS
REVERTANTS
DNA
AUTORADIOGRAPHY
GENE REPRESSORS
DNA SEQUENCING
ELECTROPHORESIS
GENE REGULATION
HELA CELLS
NUCLEOTIDES
PHOSPHORUS 32
PROTEINS
TRANSCRIPTION
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
MUTANTS
NUCLEI
NUCLEIC ACIDS
NUCLEOPROTEINS
ODD-ODD NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
VIRUSES
550201* - Biochemistry- Tracer Techniques