Antitumor efficacy of vaccinia virus-modified tumor cell vaccine
- Osaka Univ. (Japan)
The antitumor efficacies of vaccinia virus-modified tumor cell vaccines were examined in murine syngeneic MH134 and X5563 tumor cells. UV-inactivated vaccinia virus was inoculated i.p. into C3H/HeN mice that had received whole body X-irradiation at 150 rads. After 3 weeks, the vaccines were administered i.p. 3 times at weekly intervals. One week after the last injection, mice were challenged i.p. with various doses of syngeneic MH134 or X5563 viable tumor cells. Four methods were used for preparing tumor cell vaccines: X-ray irradiation; fixation with paraformaldehyde for 1 h or 3 months; and purification of the membrane fraction. All four vaccines were effective, but the former two vaccines were the most effective. A mixture of the membrane fraction of untreated tumor cells and UV-inactivated vaccinia virus also had an antitumor effect. These results indicate that vaccine with the complete cell structure is the most effective. The membrane fraction of UV-inactivated vaccinia virus-absorbed tumor cells was also effective. UV-inactivated vaccinia virus can react with not only intact tumor cells but also the purified membrane fraction of tumor cells and augment antitumor activity.
- OSTI ID:
- 6215023
- Journal Information:
- Cancer Research; (USA), Vol. 50:21; ISSN 0008-5472
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
TUMOR CELLS
VACCINES
VACCINIA VIRUS
BIOLOGICAL RADIATION EFFECTS
CELL MEMBRANES
EXPERIMENTAL NEOPLASMS
IMMUNITY
MICE
RADIATION DOSES
ULTRAVIOLET RADIATION
WHOLE-BODY IRRADIATION
X RADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
DOSES
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
IONIZING RADIATIONS
IRRADIATION
MAMMALS
MEMBRANES
MICROORGANISMS
PARASITES
RADIATION EFFECTS
RADIATIONS
RODENTS
VERTEBRATES
VIRUSES
560130* - Radiation Effects on Microorganisms
560152 - Radiation Effects on Animals- Animals