Spectrum of Bmp5 mutations from germline mutagenesis experiments in mice
- Stanford Univ. School of Medicine, Palo Alto, CA (United States); and others
Over 40 years of mutagenesis experiments using the mouse specific-locus test have produced a large number of induced germline mutations at seven loci, among them the short ear locus. We have previously shown that the short ear locus encodes bone morphogenetic protein 5 (BMP5), a member of a large family of secreted signaling molecules that play key roles in axis formation, tissue differentiation, mesenchymal-epithelial interactions, and skeletal development. Here we examine 24 chemical- and radiation-induced mutations at the short ear locus. Sequence changes in the Bmp5 open reading frame confirm the importance of cysteine residues in the function of TGF{beta} superfamily members. The spectrum of N-ethyl-N-nitrosourea-induced mutations also provides new information about the basepair, sequence context, and strand specificity of germline mutations in mammals. 52 refs., 3 figs., 2 tabs.
- DOE Contract Number:
- AC05-96OR22464
- OSTI ID:
- 621392
- Journal Information:
- Genetics, Vol. 145, Issue 2; Other Information: PBD: Feb 1997
- Country of Publication:
- United States
- Language:
- English
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