Cloning of monomeric human papillomavirus type 16 DNA integrated within cell DNA from a cervical carcinoma

Journal Article · Sun Jun 01 00:00:00 EDT 1986 · J. Virol.; (United States)

OSTI ID:6207002

Matsukura, T; Kanda, T; Furuno, A; Yoshikawa, H; Kawana, T; Yoshiike, K

The authors have molecularly cloned and characterized monomeric human papillomavirus type 16 DNA with flanking cell DNA sequences from a cervical carcinoma. Determination of nucleotide sequence around the junctions of human papillomavirus and cell DNAs revealed that at the site of integration within cell DNA the cloned viral DNA had a deletion between nucleotides 1284 and 4471 (numbering system from K. Seedorf, G. Kraemmer, M. Duerst, S. Suhai, and W.G. Roewkamp), which includes the greater part of E1 gene and the entire E2 gene. In the remaining part of the E1 gene, three guanines were found at the location where two guanines at nucleotides 1137 and 1138 have been recorded. This additional guanine shifted the reading frame and erased an interruption in the E1 gene. The data strongly suggest that, like other papillomaviruses, human papillomavirus type 16 has an uninterrupted E1 gene.

• Research Organization: National Institute of Health, Tokyo (Japan)
• OSTI ID: 6207002
• Journal Information: J. Virol.; (United States), Vol. 58:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARCINOMAS
GENETICS
ONCOGENIC VIRUSES
GENE RECOMBINATION
DNA
DNA SEQUENCING
DNA-CLONING
ELECTROPHORESIS
GENETIC MAPPING
HYBRIDIZATION
PHOSPHORUS 32
UTERUS
WOMEN
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
BODY
CLONING
DAYS LIVING RADIOISOTOPES
DISEASES
DNA HYBRIDIZATION
FEMALE GENITALS
FEMALES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MAPPING
MICROORGANISMS
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PARASITES
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
VIRUSES
550401* - Genetics- Tracer Techniques