Expression of mink cell focus-forming murine leukemia virus-related transcripts in AKR mice

Journal Article · Sun Mar 01 00:00:00 EST 1987 · J. Virol.; (United States)

OSTI ID:6204405

Khan, A S; Laigret, F; Rodi, C P

The authors used a synthetic 16-base-pair mink cell focus-forming (MCF) env-specific oligomer as radiolabeled probe to study MCF murine leukemia virus (MuLV)-related transcripts in brain, kidney, liver, spleen, and thymus tissues of AKR mice ranging from 5 weeks to 6 months (mo) of age. Tissue-specific expression of poly(A)/sup +/ RNAs was seen. In addition, all the tissues tested contained 3.0-kb messages. The transcription of these MCF-related mRNAs was independent of the presence of ecotropic and xenotropic MuLVs. In general, expression of the MCF env-related transcripts appeared to peak at 2 mo of age; these messages were barely detectable in brain, kidney, liver, and spleen tissues after 2 mo and in thymus tissue after 4 mo of age. All of the subgenomic MCF env-related mRNAs appeared to contain the 190-base-pair cellular DNA insert, characteristic of the long terminal repeats associated with endogenous MCF env-related proviruses. No genomic-size (8.4-kb) transcripts corresponding to endogenous MCF-related proviruses were detected. An 8.4-kb MCF env-related mRNA was first seen at 3 mo of age, exclusively in thymus tissue. This species most likely represents the first appearance of a recombinant MCF-related MuLV genome. The transcripts which were detected in thymus tissue might be involved in the generation of leukemogenic MCF viruses.

• Research Organization: National Institutes of Health, Bethesda, MD (USA)
• OSTI ID: 6204405
• Journal Information: J. Virol.; (United States), Vol. 61:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LEUKEMIA VIRUSES
TRANSCRIPTION
MESSENGER-RNA
TISSUE DISTRIBUTION
BRAIN
KIDNEYS
LIVER
MICE
PHOSPHORUS 32
SPLEEN
THYMUS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
GLANDS
ISOTOPES
LIGHT NUCLEI
LYMPHATIC SYSTEM
MAMMALS
MICROORGANISMS
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
ORGANS
PARASITES
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RNA
RODENTS
VERTEBRATES
VIRUSES
550701* - Microbiology- Tracer Techniques