The detection of tritium-labeled ligands and their carrier proteins using a multiwire proportional counter

Journal Article · Tue Jun 01 00:00:00 EDT 1982 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(82)90615-7· OSTI ID:6201786

Scott, B J ^[1]; Bateman, J E; Bradwell, A R

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1. Univ. of Birmingham, England

Two-dimensional immunoelectrophoresis combined with autoradiography is a powerful technique for studying the binding of radiolabeled ligands to their carrier proteins. Tritium-labeled compounds are difficult to detect by autoradiography, yet this isotope is often the radiolabel of choice, because it is possible to achieve high specific activity with no loss of biological function. Therefore an electronic detection system called a multiwire proportional counter has been investigated. This has resulted in an increase in detection speed for tritium of several thousand fold over conventional autoradiography and furthermore the results are potentially quantitative.

• OSTI ID: 6201786
• Journal Information: Anal. Biochem.; (United States), Vol. 123:1
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
LIGANDS
ELECTROPHORESIS
MULTIWIRE PROPORTIONAL CHAMBERS
SENSITIVITY
TRITIUM
RADIOCHEMICAL ANALYSIS
AUTORADIOGRAPHY
PERFORMANCE
PROTEINS
TRITIUM COMPOUNDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL ANALYSIS
HYDROGEN ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MEASURING INSTRUMENTS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROPORTIONAL COUNTERS
QUANTITATIVE CHEMICAL ANALYSIS
RADIATION DETECTORS
RADIOISOTOPES
YEARS LIVING RADIOISOTOPES
400103* - Radiometric & Radiochemical Procedures- (-1987)
440101 - Radiation Instrumentation- General Detectors or Monitors & Radiometric Instruments