Induction of UDP-glucose:salicylic acid glucosyltransferase activity in tobacco mosaic virus-inoculated tobacco (Nicotiana tabacum) leaves

Enyedi, A J; Raskin, I

Title: Induction of UDP-glucose:salicylic acid glucosyltransferase activity in tobacco mosaic virus-inoculated tobacco (Nicotiana tabacum) leaves

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Abstract

Salicylic acid (SA) is a putative signal that activates plant resistance to pathogens. SA levels increase systemically following the hypersensitive response produced by tobacco masaic virus (TMV) inoculation of tobacco (Nicotiana tabacum L. cv Xanthi-nc) leaves. The SA increase in the inoculated leaf coincided with the appearance of a [beta]-glucosidase-hydrolyzable SA conjugate identified as [beta]-O-D-glucosylsalicylic acid (GSA). SA and GSA accumulation in the TMV-inoculated leaf paralleled the increase in the activity of a UDP-glucose:salicylic acid 3-O-glucosyltransferase (EC 2.4.1.35) ([beta]-GTase) capable of converting SA to GSA. Healthy tissues had constitutive [beta]-GTase activity of 0.076 milliunits g[sup [minus]1] fresh weight. This activity started to increase 48 h after TMV inoculation, reaching its maximum (6.7-fold induction over the basal levels) 72 h after TMV inoculation. No significant GSA or elevated [beta]-GTase activity could be detected in the healthy leaf immediately above the TMV-inoculated leaf. The effect of TMV inoculation on the [beta]-GTase and GSA accumulation could be duplicated by infiltrating tobacco leaf discs with SA at the levels naturally produced in TMV-inoculated leaves (2.7--27.0 [mu]g g[sup [minus]1] fresh weight). Pretreatment of leaf discs with the protein synthesis inhibitor cycloheximide inhibited the induction of [beta]GTase by SA and prevented the formation of GSA. Ofmore »« less

Authors:

Enyedi, A J; Raskin, I ^[1]

Rutgers Univ., New Brunswick, NJ (United States)

Publication Date:: Thu Apr 01 00:00:00 EST 1993

OSTI Identifier:: 6200018

DOE Contract Number:: FG05-91ER20049

Resource Type:: Journal Article

Journal Name:: Plant Physiology; (United States)

Additional Journal Information:: Journal Volume: 101:4; Journal ID: ISSN 0032-0889

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; PLANTS; PATHOGENS; RESPONSE MODIFYING FACTORS; CYCLOHEXIMIDE; ENZYMES; GLUCOSE; GLUCOSIDASE; NICOTIANA; SALICYLIC ACID; TOBACCO; TOBACCO MOSAIC VIRUS; ALDEHYDES; ANTI-INFECTIVE AGENTS; ANTIBIOTICS; CARBOHYDRATES; CARBOXYLIC ACIDS; DRUGS; FUNGICIDES; GLYCOSYL HYDROLASES; HEXOSES; HYDROLASES; HYDROXY ACIDS; MAGNOLIOPHYTA; MAGNOLIOPSIDA; MICROORGANISMS; MONOSACCHARIDES; O-GLYCOSYL HYDROLASES; ORGANIC ACIDS; ORGANIC COMPOUNDS; PARASITES; PESTICIDES; PROTEINS; SACCHARIDES; VIRUSES; 550200* - Biochemistry

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                    Enyedi, A J, and Raskin, I. Induction of UDP-glucose:salicylic acid glucosyltransferase activity in tobacco mosaic virus-inoculated tobacco (Nicotiana tabacum) leaves.  United States: N. p., 1993. 
        Web.

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                    Enyedi, A J, & Raskin, I. Induction of UDP-glucose:salicylic acid glucosyltransferase activity in tobacco mosaic virus-inoculated tobacco (Nicotiana tabacum) leaves.  United States.

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                    Enyedi, A J, and Raskin, I. 1993.  
        "Induction of UDP-glucose:salicylic acid glucosyltransferase activity in tobacco mosaic virus-inoculated tobacco (Nicotiana tabacum) leaves".  United States.

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@article{osti_6200018,

  title        = {Induction of UDP-glucose:salicylic acid glucosyltransferase activity in tobacco mosaic virus-inoculated tobacco (Nicotiana tabacum) leaves},

  author       = {Enyedi, A J and Raskin, I},

  abstractNote = {Salicylic acid (SA) is a putative signal that activates plant resistance to pathogens. SA levels increase systemically following the hypersensitive response produced by tobacco masaic virus (TMV) inoculation of tobacco (Nicotiana tabacum L. cv Xanthi-nc) leaves. The SA increase in the inoculated leaf coincided with the appearance of a [beta]-glucosidase-hydrolyzable SA conjugate identified as [beta]-O-D-glucosylsalicylic acid (GSA). SA and GSA accumulation in the TMV-inoculated leaf paralleled the increase in the activity of a UDP-glucose:salicylic acid 3-O-glucosyltransferase (EC 2.4.1.35) ([beta]-GTase) capable of converting SA to GSA. Healthy tissues had constitutive [beta]-GTase activity of 0.076 milliunits g[sup [minus]1] fresh weight. This activity started to increase 48 h after TMV inoculation, reaching its maximum (6.7-fold induction over the basal levels) 72 h after TMV inoculation. No significant GSA or elevated [beta]-GTase activity could be detected in the healthy leaf immediately above the TMV-inoculated leaf. The effect of TMV inoculation on the [beta]-GTase and GSA accumulation could be duplicated by infiltrating tobacco leaf discs with SA at the levels naturally produced in TMV-inoculated leaves (2.7--27.0 [mu]g g[sup [minus]1] fresh weight). Pretreatment of leaf discs with the protein synthesis inhibitor cycloheximide inhibited the induction of [beta]GTase by SA and prevented the formation of GSA. Of 12 analogs of SA tested, only 2,6-dihydroxybenzoic acid induced [beta]-GTase activity. 21 refs., 5 figs.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/6200018},
  journal      = {Plant Physiology; (United States)},

  issn         = {0032-0889},

  number       = ,

  volume       = 101:4,

  place        = {United States},

  year         = {Thu Apr 01 00:00:00 EST 1993},

  month        = {Thu Apr 01 00:00:00 EST 1993}

}

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