A plant receptor-like gene, the S-locus receptor kinase of Brassica oleracea L. , encodes a functional serine/threonine kinase

Journal Article · Mon Mar 01 00:00:00 EST 1993 · Plant Physiology; (United States)

DOI:https://doi.org/10.1104/pp.101.3.1103· OSTI ID:6199943

Stein, J C; Nasrallah, J B ^[1]

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1. Cornell Univ., Ithaca, NY (United States)

To investigate the catalytic properties of the Brassica oleracea S-locus receptor kinase (SRK), the authors have expressed the domain that is homologous to protein kinases as a fusion protein in Escherichia coli. Following in vivo labeling of cultures with [sup 32]P-labeled inorganic phosphate, they observed phosphorylation of the fusion protein on serine and threonine, but not on tyrosine. In contrast, labeling was not observed when lysine-524, a residue conserved among all protein kinases, was mutated to arginine, thus confirmed that SRK phosphorylation was the result of intrinsic serine/threonine kinase activity. 26 refs., 3 figs.

• DOE Contract Number: FG02-88ER13909
• OSTI ID: 6199943
• Journal Information: Plant Physiology; (United States), Vol. 101:3; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BRASSICA
GENES
PHOSPHOTRANSFERASES
BIOLOGICAL EFFECTS
GENETIC CONTROL
ARGININE
CATABOLISM
IN VIVO
LYSINE
PHOSPHORYLATION
PROTEINS
SERINE
THREONINE
TYROSINE
AMINO ACIDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CONTROL
ENZYMES
FOOD
HYDROXY ACIDS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEST CONTROL
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
TRANSFERASES
VEGETABLES
550200* - Biochemistry
550400 - Genetics