Binding of /sup 125/I-labeled endotoxin to bovine, canine, and equine platelets and endotoxin-induced agglutination of canine platelets

Journal Article · Fri Oct 01 00:00:00 EDT 1982 · Am. J. Vet. Res.; (United States)

OSTI ID:6192051

Meyers, K M; Boehme, M; Inbar, O

Endotoxin from Escherichia coli O127:B8, Salmonella abortus-equi and S minnesota induced clumping of some canine platelets (PLT) at a final endotoxin concentration of 1 microgram/ml. Endotoxin-induced clumping of canine PLT was independent of PLT energy-requiring processes, because clumping was observed with canine PLT incubated with 2-deoxy-D-glucose and antimycin A. The PLT responded to adenosine diphosphate before, but not after, incubation with the metabolic inhibitors. Endotoxin induced a slight and inconsistant clumping of bovine and equine PLT at high (mg/ml) endotoxin concentration. High-affinity binding sites could not be demonstrated on canine, bovine, and equine PLT, using /sup 125/I-labeled E coli O127:B8 endotoxin. Nonspecific binding was observed and appeared to be due primarily to an extraneous coat on the PLT surface that was removed by gel filtration. The endotoxin that was bound to PLT did not appear to modify PLT function. An attempt to identify plasma proteins that bound physiologically relevant amounts of endotoxin was not successful. The significance of the endotoxin-induced clumping or lack of it on the pathophysiology of endotoxemia is discussed.

• Research Organization: Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology, College of Veterinary Medicine, Washington State University, Pullman
• OSTI ID: 6192051
• Journal Information: Am. J. Vet. Res.; (United States), Vol. 43:10
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
BLOOD PLATELETS
ANTIGEN-ANTIBODY REACTIONS
ENDOTOXINS
BIOLOGICAL EFFECTS
RECEPTORS
CATTLE
CHEMICAL BONDS
DOGS
ESCHERICHIA COLI
HORSES
IODINE 125
LABELLED COMPOUNDS
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BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
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ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
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TOXIC MATERIALS
TOXINS
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