Survival of density subpopulations of rabbit platelets: use of /sup 51/Cr-or /sup 111/In-labeled platelets to measure survival of least dense and most dense platelets concurrently
The origin of the density heterogeneity of platelets was studied by measuring the survival of density subpopulations of rabbit platelets separated by discontinuous Stractan density gradient centrifugation. When a total population of /sup 51/Cr-labelled platelets was injected into recipient rabbits, the relative specific radioactivity of the most dense platelets decreased rapidly. In contrast, that of the least dense platelets had not changed 24 hr after injection, and then decreased slowly. To distinguish between the possibilities that most dense platelets are cleared from the circulation more quickly than least dense platelets or that platelets decrease in density as they age in the circulation, the concurrent survival of least dense and most dense platelets, labelled with either /sup 51/Cr or /sup 111/In-labelled total platelet populations, determined concurrently in the same rabbits, are identical, calculated from 1 hr values as 100%. However, the 1-hr recovery of /sup 111/In-labelled platelets was slightly but significantly less than that of /sup 51/Cr-labelled platelets. Therefore, researchers studied the survival of /sup 51/Cr-labelled least dense and /sup 111/In-labelled most dense platelets as well as that of /sup 111/In-labelled least dense and /sup 51/Cr-labelled most dense platelets. Mean 1-hr recovery of least dense platelets, labelled with either isotope (78% +/- 7%, SD) was similar to that of most dense platelets, labelled with either isotope (77% +/- 8%; SD). Mean survival of least dense platelets was 47.3 +/- 18.7 hr (SD), which was significantly less than that of most dense platelets (76.1 +/- 21.6 hr; SD) (p less than 0.0025). These results indicate that platelets decrease in buoyant density as they age in the circulation and that most dense platelets are enriched in young platelets, and least dense in old.
- Research Organization:
- Department of Biochemistry, University of Toronto, Hamilton, Canada
- OSTI ID:
- 6188189
- Journal Information:
- Blood; (United States), Vol. 61:2
- Country of Publication:
- United States
- Language:
- English
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551001* - Physiological Systems- Tracer Techniques