Copper absorption from human milk, cow's milk, and infant formulas using a suckling rat model
Since copper deficiency is known to occur during infancy, it becomes important to assess copper uptake from various infant diets. The authors have investigated the uptake of copper from human milk, cow's milk, cow's milk formulas, cereal/milk formula and soy formula, compensating for the decay of /sup 64/Cu and using the suckling rat as a model. Radiocopper was added to the diet in trace amounts. Ultracentrifugation, ultrafiltration, and gel filtration were used to show that the added /sup 64/Cu bound to milk fractions and individual binding compounds in a manner analogous to the distribution of native copper, thus validating the use of extrinsically labeled diets. Labeled diets were intubated into 14-day-old suckling rats. Animals were killed after 6 h and tissues removed and counted. Liver copper uptake was 25% from human milk, 23% from cow's milk formula, 18% from cow's milk, 17% from premature (cow's milk based) infant formula, 17% from cereal/milk formula and 10% from soy formula. These results show that the rat pup model may provide a rapid, inexpensive, and sensitive method to assay bioavailability of copper from infant foods.
- Research Organization:
- Univ. of California, Davis
- OSTI ID:
- 6185980
- Journal Information:
- Am. J. Clin. Nutr.; (United States), Vol. 5
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effect of age on manganese uptake and retention from milk and infant formulas in rats
Effect of infant cereals on zinc and copper absorption during weaning
Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
COPPER 64
TISSUE DISTRIBUTION
UPTAKE
COPPER COMPOUNDS
BIOLOGICAL AVAILABILITY
COWS
LIVER
MILK
RATS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CATTLE
COPPER ISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
FOOD
GLANDS
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
MATERIALS
NUCLEI
ODD-ODD NUCLEI
ORGANS
RADIOISOTOPES
RODENTS
RUMINANTS
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550601 - Medicine- Unsealed Radionuclides in Diagnostics