Comparison of HLA class II genes in Caucasoid, Chinese, and Japanese patients with primary Sjoegren's syndrome
- Scripps Research Institute, La Jolla, CA (United States)
- Shanghai II Medical Univ., Shanghai (China)
- Nihon Univ. School of Medicine, Tokyo (Japan)
- Roche Molecular Systems, Alameda, CA (United States)
- Peking Union Medical Center, Beijing (China)
To better define the genetic factors that predispose to primary Sjoegren's syndrome (SS), the authors used polymerase chain reaction in combination with oligonucleotide probe hybridization and DNA sequencing to analyze HLA-DRB1, -DQA1, -DQB1, and -DPB1 alleles in Caucasoid (California), Japanese (Tokyo), and Chinese (Shanghai and Beijing) SS patients. In comparison to local controls in each region, we found: (1) increased frequency of the predicted haplotype HLA-DRB1[sup *]0301-DRB3[sup *]0101-DQA1[sup *]0501-DQB1[sup *]0201 in Caucasoid patients (p < 0.001); (2) increased frequency of the predicted haplotype HLA-DRB1[sup *]0405-DRB4[sup *]0101-DQA1[sup *]0301-DQB1[sup *]0401 in Japanese patients (p < 0.05); (3) increased frequency of the predicted haplotype DRB1[sup *]0803-DQA1[sup *]0103-DQB1[sup *]0601 in Chinese patients (p < 0.05); and (4) no statistically significant association with DBP1 alleles in any group, although an increased number of Caucasoid and Japanese SS patients possessed DPB1[sup *]0301. Comparison of DNA sequences for the three disease-associated haplotypes in these ethnic groups revealed a shared region of predicted amino acids from positions 58 to 69 in the first domain of HLA-DQB1. These results extend previous studies by demonstrating that no single class II allele was associated with 1[degrees] SS in the different ethnic groups. However, a shared amino acid motif in the DQB1 first domain was present in each disease-associated haplotype. 25 refs., 2 figs., 6 tabs.
- OSTI ID:
- 6180079
- Journal Information:
- Journal of Immunology; (United States), Vol. 150:8; ISSN 0022-1767
- Country of Publication:
- United States
- Language:
- English
Similar Records
PCR/oligonucleotide probe typing of HLA class II alleles in a Filipino population reveals an unusual distribution of HLA haplotypes
Inflammatory bowel disease associations with HLA Class II genes
Related Subjects
ANTIGENS
GENES
DNA HYBRIDIZATION
DNA SEQUENCING
LEUKOCYTES
SALIVARY GLANDS
DISEASES
CHINA
HUMAN POPULATIONS
JAPAN
SCREENING
USA
ASIA
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
DEVELOPED COUNTRIES
GLANDS
HYBRIDIZATION
MATERIALS
NORTH AMERICA
ORGANS
POPULATIONS
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics