Activation of growth factor secretion in tumorigenic states of breast cancer induced by 17. beta. -estradiol or v-Ha-ras oncogene

Journal Article · Sun Feb 01 00:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.3.837· OSTI ID:6178078

Dickson, R B; Kasid, A; Huff, K K; Bates, S E; Knabbe, C; Bronzert, D; Gelmann, E P; Lippman, M E

The MCF-7 human breast cancer cell line responds to estrogen stimulation in vitro by increased secretion of growth factors and proliferation and in vivo by tumor formation in the nude mouse. To test a possible role of growth factor secretion in expression of the tumorigenic phenotype, the authors stably transfected MCF-7 cells with the v-Ha-ras oncogene to produce the MCF-7ras cell line. The MCF-7ras cell line was tumorigenic in the absence of estrogens and secreted 3- to 5-fold elevated levels of a high molecular weight form of a type ..cap alpha.. transforming growth factor-like growth factor, type ..beta.. transforming growth factor, and insulin-like growth factor I. MCF-7ras cells, in contrast to MCF-7, were less sensitive to further growth stimulation by estrogen, type ..cap alpha.. transforming growth factor, and insulin-like growth factor I and showed little change in receptor levels for these hormones. Conditioned medium from MCF-7ras cells as well as two of its component growth factors replaced estrogen in stimulating MCF-7 colony formation in vitro. A coordinate increase in growth factor secretion by human breast cancer may contribute to its escape from estrogen dependence.

• Research Organization: National Cancer Institute, Bethesda, MD
• OSTI ID: 6178078
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:3
• Country of Publication: United States
• Language: English

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