Chlordecone impairs Na(+)-stimulated L-( sup 3 H)glutamate transport and mobility of 16-doxyl stearate in rat liver plasma membrane vesicles

Journal Article · Sat Sep 01 00:00:00 EDT 1990 · Toxicology and Applied Pharmacology; (USA)

DOI:https://doi.org/10.1016/0041-008X(90)90185-W· OSTI ID:6172762

Rochelle, L G; Miller, T L; Curtis, L R ^[1]

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1. Oregon State Univ., Corvallis (USA)

Chlordecone (CD) treatment of rat liver plasma membranes (LPM) provided in vitro evidence for mechanisms of in vivo liver dysfunction caused by CD. LPM preparations enriched 14- to 19-fold in the bile canalicular markers gamma-glutamyl transpeptidase, alkaline phosphatase, and leucine aminopeptidase were isolated from male Sprague-Dawley rats. CD inhibited the bile canalicular-specific active transport of Na(+)-stimulated L-({sup 3}H)glutamate in LPM vesicles. CD (0.08 and 0.5 mumol/mg protein) reduced both the initial velocity and the maximum level of Na(+)-stimulated L-(3H)glutamate uptake without significantly reducing Na(+)-independent uptake. In vitro treatment of LPM with CD (0.2-1.0 mumols/mg protein) also reduced the mobility of a 16-doxyl stearate spin label probe in a concentration-dependent manner. No change in mobility was apparent at CD concentrations below 0.2 mumol/mg protein. These results demonstrated that CD impaired a bile canalicular-specific transport system and induced liver plasma membrane perturbation. Na(+)-stimulated L-({sup 3}H)glutamate uptake was more sensitive to CD than was detectable immobilization of the spin label probe.

• OSTI ID: 6172762
• Journal Information: Toxicology and Applied Pharmacology; (USA), Vol. 105:2; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUTAMIC ACID
MEMBRANE TRANSPORT
INSECTICIDES
BIOLOGICAL EFFECTS
BIOLOGICAL MARKERS
CELL MEMBRANES
ELECTRON SPIN RESONANCE
FRACTIONATION
INHIBITION
LIVER
RATS
SODIUM COMPOUNDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALI METAL COMPOUNDS
AMINO ACIDS
ANIMALS
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DIGESTIVE SYSTEM
GLANDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAGNETIC RESONANCE
MAMMALS
MEMBRANES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PESTICIDES
RESONANCE
RODENTS
SEPARATION PROCESSES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques