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Title: Characterization of the detergent solubilized receptor for gastrin-releasing peptide

Abstract

Properties of detergent solubilized gastrin-releasing peptide receptor were investigated. Swiss 3T3 membranes were covalently labeled with ({sup 125}I)GRP and homobifunctional cross-linkers. A major labeled protein of 75 kDa was resolved using SDS-polyacrylamide gel electrophoresis. When the same preparation was solubilized with zwitterionic detergent and analyzed under nondenaturing conditions the protein bound radioactivity was resolved in two different peaks, a major one of apparent molecular weight 220,000 (peak 1) and a minor one of 80,000 (peak 2) both containing the 75 kDa protein. Specific ligand binding activity also eluted with peak 1. These results indicate that the active form of bombesin/GRP receptor is a large complex containing the 75 kDa ligand binding domain.

Authors:
; ; ; ; ;  [1]
  1. Rorer Biotechnology, Inc., King of Prussia, PA (USA)
Publication Date:
OSTI Identifier:
6171826
Resource Type:
Journal Article
Journal Name:
Peptides (Fayetteville, New York); (USA)
Additional Journal Information:
Journal Volume: 11:4; Journal ID: ISSN 0196-9781
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PEPTIDES; RECEPTORS; CHEMICAL COMPOSITION; BIOCHEMICAL REACTION KINETICS; CELL MEMBRANES; DETERGENTS; ELECTROPHORESIS; IODINE 125; MOLECULAR WEIGHT; REAGENTS; SOLUBILITY; TRACER TECHNIQUES; ADDITIVES; BETA DECAY RADIOISOTOPES; CELL CONSTITUENTS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; EMULSIFIERS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; MEMBRANE PROTEINS; MEMBRANES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PROTEINS; RADIOISOTOPES; REACTION KINETICS; SURFACTANTS; WETTING AGENTS; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Cirillo, D, Naldini, L, Moody, T W, Comoglio, P, Schlessinger, J, and Kris, R. Characterization of the detergent solubilized receptor for gastrin-releasing peptide. United States: N. p., 1990. Web. doi:10.1016/0196-9781(90)90189-C.
Cirillo, D, Naldini, L, Moody, T W, Comoglio, P, Schlessinger, J, & Kris, R. Characterization of the detergent solubilized receptor for gastrin-releasing peptide. United States. https://doi.org/10.1016/0196-9781(90)90189-C
Cirillo, D, Naldini, L, Moody, T W, Comoglio, P, Schlessinger, J, and Kris, R. 1990. "Characterization of the detergent solubilized receptor for gastrin-releasing peptide". United States. https://doi.org/10.1016/0196-9781(90)90189-C.
@article{osti_6171826,
title = {Characterization of the detergent solubilized receptor for gastrin-releasing peptide},
author = {Cirillo, D and Naldini, L and Moody, T W and Comoglio, P and Schlessinger, J and Kris, R},
abstractNote = {Properties of detergent solubilized gastrin-releasing peptide receptor were investigated. Swiss 3T3 membranes were covalently labeled with ({sup 125}I)GRP and homobifunctional cross-linkers. A major labeled protein of 75 kDa was resolved using SDS-polyacrylamide gel electrophoresis. When the same preparation was solubilized with zwitterionic detergent and analyzed under nondenaturing conditions the protein bound radioactivity was resolved in two different peaks, a major one of apparent molecular weight 220,000 (peak 1) and a minor one of 80,000 (peak 2) both containing the 75 kDa protein. Specific ligand binding activity also eluted with peak 1. These results indicate that the active form of bombesin/GRP receptor is a large complex containing the 75 kDa ligand binding domain.},
doi = {10.1016/0196-9781(90)90189-C},
url = {https://www.osti.gov/biblio/6171826}, journal = {Peptides (Fayetteville, New York); (USA)},
issn = {0196-9781},
number = ,
volume = 11:4,
place = {United States},
year = {Sun Jul 01 00:00:00 EDT 1990},
month = {Sun Jul 01 00:00:00 EDT 1990}
}