The biphasic effect of triiodothyronine compared to bone resorbing effect of PTH on bone modelling of mouse long bone in vitro

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Bone; (USA)

DOI:https://doi.org/10.1016/8756-3282(90)90084-C· OSTI ID:6171281

Soskolne, W A; Schwartz, Z; Goldstein, M; Ornoy, A ^[1]

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1. Hebrew Univ. Hadassah, Jerusalem (Israel)

To examine the effects of T3 on fetal long bone modelling the radii and ulnae of 16 day old fetal mice were grown in vitro for two days. Their growth, mineralization, and resorption were assessed by measuring diaphyseal length, calcium and phosphorus content, hydroxyproline content, and the release of incorporated {sup 45}Ca. The effects of T3 were compared to the effects of 1-34 PTH, a known resorbing agent, on the same system. Devitalized bones were used as a control. The results showed that T3 had a biphasic effect. At high concentrations (10(-5) M-10(-6) M) T3 inhibited the growth of the bones as indicated by their diaphyseal length and hydroxyproline content. Calcium and phosphorus content were significantly decreased while {sup 45}Ca release was increased. Similar effects were also found after the addition of 1-34 PTH to the media. However, T3, at lower concentrations (10(-7) M-10(-9) M), stimulated the growth and calcification of the bones as indicated by an increase in diaphyseal length and the hydroxyproline, calcium, and phosphorus content. {sup 45}Ca release was significantly decreased at these concentrations. Neither T3 nor 1-34 PTH affected devitalized bones in the same system. The results suggest that at physiological concentrations, T3 has a direct, anabolic effect on bone, which may explain its major role in the growth process of various species. At high doses, however, T3 stimulates bone resorption in a way similar to PTH.

• OSTI ID: 6171281
• Journal Information: Bone; (USA), Vol. 11:5; ISSN 8756-3282
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PARATHORMONE
BIOLOGICAL EFFECTS
SKELETON
ANIMAL GROWTH
TRIIODOTHYRONINE
BODY COMPOSITION
CALCIUM
CALCIUM 45
COMPARATIVE EVALUATIONS
HYDROXYPROLINE
IN VITRO
MICE
PHOSPHORUS
TISSUE CULTURES
TRACER TECHNIQUES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
AMINES
AMINO ACIDS
ANIMALS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CALCIUM ISOTOPES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
GROWTH
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROXY ACIDS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
METALS
NONMETALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDE HORMONES
PYRROLES
PYRROLIDINES
RADIOISOTOPES
RODENTS
THYROID HORMONES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques