Alterations in phosphate metabolism during cellular recovery of radiation damage in yeast
Alterations were examined in phosphate pools during cellular recovery from radiation damage in intact, wild-type diploid yeast cells using phosphorus 31 nuclear magnetic resonance (NMR) spectroscopy. Concurrent cell survival analysis was determined following exposure to cobalt 60 gamma radiation. Cells held in citrate-buffered saline (CBS) showed increased survival with increasing time after irradiation (liquid holding recovery, LHR) with no further recovery beyond 48 h. Addition of 100 mmol/cu. dm glucose to the recovery medium resulted in greater recovery. In the presence of 5 mmol/cu. dm 2-deoxyglucose (2-DG), LHR was completely inhibited. ATP was observable by NMR only when glucose was present in the recovery medium. In control cells, ATP concentrations increased and plateaued with increasing recovery time. With increasing radiation dose, the increase in ATP was of lesser magnitude, and after 2000 Gy no increase was observed. The observations suggest that either the production of ATP in irradiated cells is suppressed, or there is enhanced ATP utilization for repair of radiation damage. In CBS with 100 mmol/cu. dm glucose, a dose-dependent decrease in polyphosphate (polyP) was detectable with no concurrent increase in inorganic phosphate (p sub i). When 2-DG was present in the recovery medium, polyP decreased, but there was a simultaneous increase in p sub i with increasing radiation dose and recovery time. This suggests that the polyP are hydrolyzed as a source of phosphates for repair of radiation damage.
- Research Organization:
- Armed Forces Radiobiology Research Inst., Bethesda, MD (USA)
- OSTI ID:
- 6171151
- Report Number(s):
- AD-A-202515/3/XAB; AFRRI-SR-88-22
- Resource Relation:
- Other Information: Pub. in International Jnl. of Radiation, Vol. 54, No. 4, 545-562(1988)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
PHOSPHATES
METABOLISM
PHOSPHORUS 31
NMR SPECTRA
YEASTS
BIOLOGICAL RADIATION EFFECTS
CONTROL
CYTOLOGY
GLUCOSE
INORGANIC COMPOUNDS
IRRADIATION
PRODUCTION
RECOVERY
REPAIR
SPECTROSCOPY
ALDEHYDES
BIOLOGICAL EFFECTS
BIOLOGY
CARBOHYDRATES
EUMYCOTA
FUNGI
HEXOSES
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PLANTS
RADIATION EFFECTS
SACCHARIDES
SPECTRA
STABLE ISOTOPES
560162* - Radionuclide Effects
Kinetics
& Toxicology- Animals
Plants
Microorganisms
& Cells
550700 - Microbiology