Contractile properties of isolated vascular smooth muscle after photoradiation

Freas, W; Hart, J L; Golightly, D; McClure, H; Muldoon, S M

Title: Contractile properties of isolated vascular smooth muscle after photoradiation

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Abstract

The purpose of this study was to characterize the responses of various types of vascular smooth muscle to conditions that would be encountered during photodynamic therapy, namely laser illumination of photosensitizer-pretreated tissue. Vascular smooth muscle obtained from representative canine, rodent, and rabbit vascular beds was cut into rings and placed in organ baths (37 degrees C, aerated with 95% O2-5% CO2). These vessels were pretreated for 30 min with the photosensitizer hematoporphyrin derivative (HpD, 3-30 micrograms/ml) washed, and then exposed to red laser light (633 nm, 1-3.5 mW) for up to 20 min. Under basal tension conditions laser illumination of HpD-pretreated vessels resulted in an increase in tension, whereas laser illumination of vessels not exposed to HpD did not contract. This sustained contraction was not reversed by washing the tissue with fresh Krebs-Ringer solution. Responses to norepinephrine, transmural electrical stimulation, and elevated concentrations of KCl were reduced in blood vessels tested after HpD laser illumination. Laser-induced contractions of canine carotid arteries did not require the presence of an intact vascular endothelium. Vascular effect of these photosensitizers appears to involve the formation of oxygen-derived radicals. This preparation could provide a good model for examining the effects of free radicals on vascularmore » physiology.« less

Authors:: Freas, W; Hart, J L; Golightly, D; McClure, H; Muldoon, S M

Publication Date:: Wed Mar 01 00:00:00 EST 1989

Research Org.:: Uniformed Services Univ. of the Health Sciences, Bethesda, MD (USA)

OSTI Identifier:: 6170356

Resource Type:: Journal Article

Journal Name:: Am. J. Physiol.; (United States)

Additional Journal Information:: Journal Volume: 256

Country of Publication:: United States

Language:: English

Subject:: 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LASER RADIATION; BIOLOGICAL EFFECTS; MUSCLES; CONTRACTION; RADICALS; BIOSYNTHESIS; ARTERIES; ASCORBIC ACID; CATALASE; DOGS; HEMATOPORPHYRINS; IN VITRO; LASERS; RABBITS; SUPEROXIDE DISMUTASE; VEINS; ANIMALS; BLOOD VESSELS; BODY; CARBOXYLIC ACIDS; CARDIOVASCULAR SYSTEM; ELECTROMAGNETIC RADIATION; ENZYMES; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; MAMMALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; OXIDOREDUCTASES; PEROXIDASES; PIGMENTS; PORPHYRINS; RADIATIONS; SYNTHESIS; VERTEBRATES; VITAMINS; 560400* - Other Environmental Pollutant Effects

Citation Formats


                    Freas, W, Hart, J L, Golightly, D, McClure, H, and Muldoon, S M. Contractile properties of isolated vascular smooth muscle after photoradiation.  United States: N. p., 1989. 
        Web.

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                    Freas, W, Hart, J L, Golightly, D, McClure, H, & Muldoon, S M. Contractile properties of isolated vascular smooth muscle after photoradiation.  United States.

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                    Freas, W, Hart, J L, Golightly, D, McClure, H, and Muldoon, S M. 1989.  
        "Contractile properties of isolated vascular smooth muscle after photoradiation".  United States.

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@article{osti_6170356,

  title        = {Contractile properties of isolated vascular smooth muscle after photoradiation},

  author       = {Freas, W and Hart, J L and Golightly, D and McClure, H and Muldoon, S M},

  abstractNote = {The purpose of this study was to characterize the responses of various types of vascular smooth muscle to conditions that would be encountered during photodynamic therapy, namely laser illumination of photosensitizer-pretreated tissue. Vascular smooth muscle obtained from representative canine, rodent, and rabbit vascular beds was cut into rings and placed in organ baths (37 degrees C, aerated with 95% O2-5% CO2). These vessels were pretreated for 30 min with the photosensitizer hematoporphyrin derivative (HpD, 3-30 micrograms/ml) washed, and then exposed to red laser light (633 nm, 1-3.5 mW) for up to 20 min. Under basal tension conditions laser illumination of HpD-pretreated vessels resulted in an increase in tension, whereas laser illumination of vessels not exposed to HpD did not contract. This sustained contraction was not reversed by washing the tissue with fresh Krebs-Ringer solution. Responses to norepinephrine, transmural electrical stimulation, and elevated concentrations of KCl were reduced in blood vessels tested after HpD laser illumination. Laser-induced contractions of canine carotid arteries did not require the presence of an intact vascular endothelium. Vascular effect of these photosensitizers appears to involve the formation of oxygen-derived radicals. This preparation could provide a good model for examining the effects of free radicals on vascular physiology.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/6170356},
  journal      = {Am. J. Physiol.; (United States)},
number       = ,

  volume       = 256,

  place        = {United States},

  year         = {Wed Mar 01 00:00:00 EST 1989},

  month        = {Wed Mar 01 00:00:00 EST 1989}

}

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