Possible mechanism for preterm labor associated with bacterial infection. I. Stimulation of phosphoinositide metabolism by endotoxin in endometrial fibroblasts

Journal Article · Sun Jul 01 00:00:00 EDT 1990 · Research Communications in Chemical Pathology and Pharmacology; (USA)

OSTI ID:6168865

Khan, A A; Imai, A; Tamaya, T ^[1]

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1. Gifu Univ. School of Medicine (Japan)

Growing evidence suggests an association between intra-amniotic infection and premature initiation of parturition. We recently demonstrated that some factor(s) including endotoxin produced by the organism stimulates endogenous phospholipase A2 resulting in liberation of arachidonic acid and prostaglandin formation. The studies presented in this report were designated to evaluate the mechanism for endotoxin to stimulate phospholipase A2 using human endometrial fibroblasts. Exposure of the fibroblasts to endotoxin from Escherichia coli in the presence of ({sup 32}P) phosphate increased {sup 32}P-labeling of phosphatidic acid (PA) and phosphatidyl-inositol (PI) in a dose-dependent and a time-dependent manners. The PA labeling occurred without a measurable lag time. These findings demonstrate that the endotoxin stimulates phosphoinositide metabolism in human endometrial fibroblasts by a receptor-mediated mechanism. Membrane phosphoinositide turnover stimulated by endotoxin results in cytosolic Ca{sup 2+} increment, liberation of arachidonic acid, which may be involved in the initiation of parturition.

• OSTI ID: 6168865
• Journal Information: Research Communications in Chemical Pathology and Pharmacology; (USA), Vol. 69:1; ISSN 0034-5164
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ARACHIDONIC ACID
BIOSYNTHESIS
BACTERIAL DISEASES
BIOLOGICAL EFFECTS
PHOSPHOLIPIDS
METABOLISM
PROSTAGLANDINS
DOSE-RESPONSE RELATIONSHIPS
FIBROBLASTS
LIPASES
MAN
PHOSPHORUS 32
PREGNANCY
TIME DEPENDENCE
TOXINS
TRACER TECHNIQUES
UTERUS
ANIMAL CELLS
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
ENZYMES
ESTERASES
ESTERS
FEMALE GENITALS
HYDROLASES
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAMMALS
MATERIALS
MONOCARBOXYLIC ACIDS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
SOMATIC CELLS
SYNTHESIS
TOXIC MATERIALS
VERTEBRATES
550901* - Pathology- Tracer Techniques