Regulated bioluminescence as a tool for bioremediation process monitoring and control of bacterial cultures
- Oak Ridge National Lab., TN (United States)
- Tennessee Univ., Knoxville, TN (United States). Center for Environmental Biotechnology
An effective on-line monitoring technique for toxic waste bioremediation using bioluminescent microorganisms has demonstrated great potential for the description and optimization of biological processes. The lux genes of the bacterium Vibrio Fascheri are used by this species to produce visible light. The lux genes can be genetically fused to the control region of a catabolic gene, with the result that bioluminescence is produced whenever the catabolic gene is induced. Thus the detection of light from a sample (monoculture, consortium, or bioreactor) indicates that genetic expression from a specific gene is occurring. We have used this technique to monitor biodegradation of specific contaminants from waste sites. For these studies, fusions between the lux genes and the operons for naphthalene (nah) and toluene/xylene (xyl) degradation were constructed. Strains carrying one of these fusions respond sensitively and specifically to target substrates. Bioluminescence from these cultures can be rapidly measured in a non-destructive and non-invasive manner. The potential for this technique in this and other biological systems is discussed. 7 refs., 3 figs.
- Research Organization:
- Oak Ridge National Lab., TN (United States)
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 6162503
- Report Number(s):
- CONF-911231-1; ON: DE92002707
- Resource Relation:
- Conference: Technology 2000, San Jose, CA (United States), 3-5 Dec 1991
- Country of Publication:
- United States
- Language:
- English
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Catabolic gene expression is monitored by bioluminescence in bioreactor studies
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Related Subjects
BIOLUMINESCENCE
BIOREACTORS
MONITORING
PSEUDOMONAS
GENETIC ENGINEERING
TOXIC MATERIALS
BIODEGRADATION
BIOCHEMISTRY
BIOTECHNOLOGY
GENE OPERONS
GENE REGULATION
NAPHTHALENE
TOLUENE
XYLENES
ALKYLATED AROMATICS
AROMATICS
BACTERIA
CHEMICAL REACTIONS
CHEMISTRY
CONDENSED AROMATICS
DECOMPOSITION
HYDROCARBONS
LUMINESCENCE
MATERIALS
MICROORGANISMS
ORGANIC COMPOUNDS
550700* - Microbiology
550200 - Biochemistry
550400 - Genetics
550500 - Metabolism