DNA/DNA in situ hybridization with enzyme linked probes
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6157518
A non-radioactive in situ nucleic acid hybridization method which requires no antibodies, haptens, avidin or biotin intermediateries is presented. Horseradish peroxidase (HRP) labeled nucleic acid probes are hybridized in situ for 2 hours or less, followed by brief washing of hybridized cells and the direct detection of in situ hybrids with diaminobenzidine (DAB). Application of this method to the detection of Human Papilloma Virus (HPV) in human cells is shown.
- OSTI ID:
- 6157518
- Report Number(s):
- CONF-870644-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
Similar Records
Hairpin DNA Switch for Ultrasensitive Spectrophotometric Detection of DNA Hybridization Based on Gold Nanoparticles and Enzyme Signal Amplification
Comparison of peroxidase-labeled DNA probes with radioactive RNA probes for detection of human papillomaviruses by in situ hybridization in paraffin sections
Human papillomavirus type 16 DNA in periungual squamous cell carcinomas
Journal Article
·
Sun Aug 01 00:00:00 EDT 2010
· Analytical Chemistry
·
OSTI ID:6157518
+3 more
Comparison of peroxidase-labeled DNA probes with radioactive RNA probes for detection of human papillomaviruses by in situ hybridization in paraffin sections
Journal Article
·
Tue Jan 01 00:00:00 EST 1991
· Modern Pathology; (USA)
·
OSTI ID:6157518
+1 more
Human papillomavirus type 16 DNA in periungual squamous cell carcinomas
Journal Article
·
Fri May 12 00:00:00 EDT 1989
· JAMA, Journal of the American Medical Association; (USA)
·
OSTI ID:6157518
+3 more