Use of deuterium labelled glucose in evaluating the pathway of hepatic glycogen synthesis

Journal Article · Wed Mar 15 00:00:00 EST 1989 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(89)90024-7· OSTI ID:6154252

Goodman, M N; Masuoka, L K; deRopp, J S; Jones, A D

Deuterium labelled glucose has been used to study the pathway of hepatic glycogen synthesis during the fasted-refed transition in rats. Deuterium enrichment of liver glycogen was determined using nuclear magnetic resonance as well as mass spectroscopy. Sixty minutes after oral administration of deuterated glucose to fasted rats, the portal vein blood was fully enriched with deuterated glucose. Despite this, less than half of the glucose molecules incorporated into liver glycogen contained deuterium. The loss of deuterium label from glucose is consistent with hepatic glycogen synthesis by an indirect pathway requiring prior metabolism of glucose. The use of deuterium labelled glucose may prove to be a useful probe to study hepatic glycogen metabolism. Its use may also find application in the study of liver glycogen metabolism in humans by a noninvasive means.

• Research Organization: Davis School of Medicine, Sacramento, CA (USA)
• OSTI ID: 6154252
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 159:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLYCOGEN
METABOLISM
BIOLOGICAL PATHWAYS
BLOOD
DEUTERIUM
FASTING
GLUCOSE
LIVER
MASS SPECTROSCOPY
NUCLEAR MAGNETIC RESONANCE
ORAL ADMINISTRATION
RATS
TRACER TECHNIQUES
ALDEHYDES
ANIMALS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CARBOHYDRATES
DIGESTIVE SYSTEM
GLANDS
HEXOSES
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
MATERIALS
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
POLYSACCHARIDES
RESONANCE
RODENTS
SACCHARIDES
SPECTROSCOPY
STABLE ISOTOPES
VERTEBRATES
550501* - Metabolism- Tracer Techniques