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Title: Toluene model for hydrocarbon risk assessment. Final report, 1 January-31 December 1989

Abstract

This project was for continuation of research to investigate the molecular mode of action of a membrane-active hydrocarbon, toluene, potentially present in the Air Force environment as a flight fuel component or from other sources and to serve as a model for other membrane-active molecules in the environment. Two important target sites were identified where rapid dose-dependent but reversible changes in the membrane organization occurred at low dose levels. One of these was at the plasma membrane where the ability of the membrane to form protuberances was severely compromised. The other concerned a failure to form protuberances by membranes involved in internal trafficking between the endoplasmic reticulum and the Golgi apparatus. This step was reproduced in a cell-free system making detailed studies possible. The toluene inhibited step was identified as dependent on ATP hydrolysis. The involved ATPase activity was characterized, solubilized and partially purified.

Authors:
Publication Date:
Research Org.:
Purdue Univ., Lafayette, IN (USA). Dept. of Medicinal Chemistry and Pharmacognosy
OSTI Identifier:
6154014
Report Number(s):
AD-A-225358/1/XAB
CNN: AFOSR-89-0219
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ATP-ASE; ENZYME ACTIVITY; TOLUENE; TOXICITY; ATP; CELL MEMBRANES; DOSE-RESPONSE RELATIONSHIPS; MAN; PROGRESS REPORT; RISK ASSESSMENT; ACID ANHYDRASES; ALKYLATED AROMATICS; ANIMALS; AROMATICS; CELL CONSTITUENTS; DOCUMENT TYPES; ENZYMES; HYDROCARBONS; HYDROLASES; MAMMALS; MEMBRANES; NUCLEOTIDES; ORGANIC COMPOUNDS; PHOSPHOHYDROLASES; PRIMATES; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Morre, J. Toluene model for hydrocarbon risk assessment. Final report, 1 January-31 December 1989. United States: N. p., 1990. Web.
Morre, J. Toluene model for hydrocarbon risk assessment. Final report, 1 January-31 December 1989. United States.
Morre, J. 1990. "Toluene model for hydrocarbon risk assessment. Final report, 1 January-31 December 1989". United States.
@article{osti_6154014,
title = {Toluene model for hydrocarbon risk assessment. Final report, 1 January-31 December 1989},
author = {Morre, J},
abstractNote = {This project was for continuation of research to investigate the molecular mode of action of a membrane-active hydrocarbon, toluene, potentially present in the Air Force environment as a flight fuel component or from other sources and to serve as a model for other membrane-active molecules in the environment. Two important target sites were identified where rapid dose-dependent but reversible changes in the membrane organization occurred at low dose levels. One of these was at the plasma membrane where the ability of the membrane to form protuberances was severely compromised. The other concerned a failure to form protuberances by membranes involved in internal trafficking between the endoplasmic reticulum and the Golgi apparatus. This step was reproduced in a cell-free system making detailed studies possible. The toluene inhibited step was identified as dependent on ATP hydrolysis. The involved ATPase activity was characterized, solubilized and partially purified.},
doi = {},
url = {https://www.osti.gov/biblio/6154014}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 29 00:00:00 EDT 1990},
month = {Tue May 29 00:00:00 EDT 1990}
}

Technical Report:
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