Endonucleases involved in repair and recombination of DNA
When our DOE support began as a contract in 1970, from the AEC, it was our intent to begin to understand how several enzymes which we had detected in E. coli might be involved in DNA recombination and repair. These studies led to our characterization of the recBC DNase (exonuclease 5) as well as endonucleases 3 and 5. As research supported by that contract progressed, we expanded our interests to include mammalian enzymes involved in base excision repair, most notably AP endonucleases, DNA glycosylases and DNA purine insertase. A logical next step involved the inclusion of DNA polymerases into our studies of repair. Current progress includes research on: isolation of xeroderma pigmentosum correction factors; isolation of ultraviolet (UV) endonucleases; mitochondrial repair enzymes; alkylation damage repair; comparisons of repair in normal diploid, transformed, and non-mitotic cells; and repair reactions by DNA polymerases.
- Research Organization:
- California Univ., Berkeley, CA (USA). Dept. of Biochemistry
- Sponsoring Organization:
- DOE/ER
- DOE Contract Number:
- FG03-86ER60395
- OSTI ID:
- 6153063
- Report Number(s):
- DOE/ER/60395-T2; ON: DE91008269
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA REPAIR
MOLECULAR BIOLOGY
ENDONUCLEASES
ENZYME ACTIVITY
GENE RECOMBINATION PROTEINS
ULTRAVIOLET RADIATION
RADIATION EFFECTS
XERODERMA PIGMENTOSUM
DNA
DNA POLYMERASES
ESCHERICHIA COLI
FIBROBLASTS
GENE RECOMBINATION
HELA CELLS
MAMMALS
MITOCHONDRIA
PROGRESS REPORT
ANIMAL CELLS
ANIMALS
BACTERIA
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
DISEASES
DNA-ASE
DOCUMENT TYPES
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
HYDROLASES
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOPROTEINS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHODIESTERASES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PROTEINS
RADIATIONS
RECOVERY
REPAIR
SKIN DISEASES
SOMATIC CELLS
TRANSFERASES
VERTEBRATES
550200* - Biochemistry
560120 - Radiation Effects on Biochemicals
Cells
& Tissue Culture