Isolation of a urinary digitalis-like factor indistinguishable from digoxin
- Univ. of Tokyo (Japan)
A digitalis-like factor has been purified to apparent homogeneity from human urine based on the inhibitory effect on ({sup 3}H) ouabain binding to intact human erythrocytes. The purification scheme involved large scale adsorption followed by preparative, semipreparative and analytical high-performance liquid chromatography. The purified material showed a prominent digoxin-like immunoreactivity. The behaviour of the isolated substance was identical to that of authentic digoxin in three high-performance liquid chromatography and three thin-layer chromatography systems. Moreover, fast atom bombardment mass spectrum and proton nuclear magnetic resonance spectrum suggested that the purified material may be indistinguishable from digoxin.
- OSTI ID:
- 6152878
- Journal Information:
- Biochemical and Biophysical Research Communications; (USA), Vol. 173:3; ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DIGOXIN
FRACTIONATION
OUABAIN
BIOCHEMICAL REACTION KINETICS
ERYTHROCYTES
IMMUNE REACTIONS
LIQUID COLUMN CHROMATOGRAPHY
MAN
MASS SPECTROSCOPY
NMR SPECTRA
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URINE
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHROMATOGRAPHY
DIGITALIS GLYCOSIDES
DRUGS
GLYCOSIDES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
PRIMATES
REACTION KINETICS
SEPARATION PROCESSES
SPECTRA
SPECTROSCOPY
STROPHANTHINS
VERTEBRATES
WASTES
550201* - Biochemistry- Tracer Techniques