Distribution of radiolabeled endotoxin with particular reference to the eye: concise communication
A single systemic injection of endotoxin (lipopolysaccharide or LPS) reproducibly induces a cellular infiltrate in the uveal tract of the rat eye within 24 hr. Other organs are not comparably sensitive to systemic endotoxin. One hypothesis to explain this unique sensitivity is that endotoxin is preferentially bound by ocular tissue. Researchers tested this hypothesis by studying the distribution in the rat of intravenously injected endotoxin that had been radiolabeled with /sup 99m/Tc or /sup 32/P. With either radionuclide the concentration of endotoxin per gram of tissue at a variety of times after injection ranging from 5 min to 3 hr and 45 min, was markedly less in the eye than in liver, kidney, or spleen. A study with radiolabeled albumin indicated that these differences could not be ascribed solely to the organ's blood volume. They demonstrate, therefore, that the eye does not preferentially bind endotoxin, and they are compatible with the hypothesis that endotoxin's ocular effects are indirectly mediated.
- Research Organization:
- Stanford Medical Center, California
- OSTI ID:
- 6148987
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 24:1
- Country of Publication:
- United States
- Language:
- English
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ISOMERIC NUCLEI
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PHYSIOLOGY
RADIONUCLIDE KINETICS
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ISOTOPE APPLICATIONS
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ODD-ODD NUCLEI
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550501* - Metabolism- Tracer Techniques