An assessment of the immunofluorescence technique as a method for demonstrating the histological localization of tetrahydrocannabinol in mammalian tissues

Journal Article · Fri Oct 01 00:00:00 EDT 1982 · J. Forensic Sci.; (United States)

DOI:https://doi.org/10.1520/JFS12200J· OSTI ID:6148957

Morley, M; Gee, D J

The use of the indirect immunofluorescence technique as a method for demonstrating the histological localization of tetrahydrocannabinol (delta-THC) has been examined. The experimental protocol was designed in order that optimal staining conditions with respect to temperature, the length of time of incubations and washes, and the dilution of the antisera should be defined. No marked differences were detected between frozen sections of liver from normal and delta-THC-injected mice. Results from radiotracer experiments using human liver suggest that the success of the method is dependent upon the solubility characteristics of the antigen-antibody complex.

• Research Organization: Department of Forensic Medicine, University of Leeds, Leeds, United Kingdom
• OSTI ID: 6148957
• Journal Information: J. Forensic Sci.; (United States), Vol. 27:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MARIHUANA
METABOLISM
TISSUE DISTRIBUTION
ANTIGEN-ANTIBODY REACTIONS
BIOLOGICAL LOCALIZATION
FLUORESCENCE
HISTOLOGY
IMMUNE SERUMS
LIVER
TRACER TECHNIQUES
TRITIUM COMPOUNDS
BODY
DIGESTIVE SYSTEM
DISTRIBUTION
GLANDS
HERBS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LUMINESCENCE
ORGANS
PLANTS
550501* - Metabolism- Tracer Techniques