Recovery from severe hematopoietic suppression using recombinant human granulocyte-macrophage colony-stimulating factor
The ability of recombinant human granulocytemacrophage colony-stimulating factor (rhGM-CSF) to enhance recovery of a radiation-suppressed hematopoietic system was evaluated in a nonuniform radiation-exposure model using the rhesus monkey. Recombinant human GM-CSF treatment for 7 days after a lethal, nonuniform radiation exposure of 800 cGy was sufficient to enhance hematopoietic reconstitution, leading to an earlier recovery. Monkeys were treated with 72,000 U/kg/day of rhGm-CSF delivered continuously through an Alzet mini-osmotic pump implanted subcutaneously on day 3. Treated monkeys demonstrated effective granulocyte and platelet levels in the peripheral blood, 4 and 7 days earlier, respectively, than control monkeys. Granulocyte-macrophage colony-forming unit (CFU-GM) activity in the bone marrow was monitored to evaluate the effect of rhGM-CSF on marrow recovery. Treatment with rhGM-CSF led to an early recovery of CFU-GM activity suggesting that rhGM-CSF acted on an earlier stem cell population to generate CFU-GM. Thus, the effect of rhGM-CSF on hematopoietic regeneration, granulocyte recovery, and platelet recovery are evaluated.
- Research Organization:
- Naval Medical Research Inst., Bethesda, MD (USA)
- OSTI ID:
- 6148932
- Report Number(s):
- AD-A-202416/4/XAB; NMRI-88-43
- Resource Relation:
- Other Information: Pub. in Experimental Hematology, Vol. 16, 344-348(1988)
- Country of Publication:
- United States
- Language:
- English
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560150* - Radiation Effects on Animals