Use of microbial spores as a biocatalyst
- Kyoto Univ. (Japan). Research Inst. for Food Science
Endospores of a bacterium Bacillus subtilis and ascospores of a yeast Saccharomyces cerevisiae contained almost all the activities for the same enzymes as vegetative cells. The biotechnological potential of spores was studied by selecting adenosine 5[prime]-triphosphatase and alkaline phosphatase in bacterial and yeast spores, respectively, as model enzymes. The activity of both enzymes was efficiently expressed when the spores were treated by physical (sonication or electric field pulse) and chemical (organic solvents or detergents) methods. The yeast spores were immobilized in polyacrylamide gel without any appreciable loss of activity. The immobilized spores were packed in a column and used successfully for the continuous reactions of alkaline phosphatase and glyoxalase I. The microbial spores were confirmed to be promising as a biocatalyst for the production of useful chemicals in bioreactor systems.
- OSTI ID:
- 6146800
- Journal Information:
- CRC Critical Reviews in Biotechnology; (United States), Vol. 13:3; ISSN 0738-8551
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
BACILLUS SUBTILIS
BIOTECHNOLOGY
BACTERIAL SPORES
SENSITIVITY
PHOSPHATASES
ENZYME ACTIVITY
SACCHAROMYCES CEREVISIAE
BIOREACTORS
BACILLUS
BACTERIA
ENZYMES
ESTERASES
EUMYCOTA
FUNGI
HYDROLASES
MICROORGANISMS
ORGANIC COMPOUNDS
PLANTS
PROTEINS
SACCHAROMYCES
SPORES
YEASTS
550200* - Biochemistry
090900 - Biomass Fuels- Processing- (1990-)