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Title: Modulation of DL-. alpha. -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid/quisqualate receptors by phospholipase A sub 2 : A necessary step in long-term potentiation

Abstract

The effects of kainate (KA)-induced epileptic seizures on the binding properites of hippocampal glutamate receptors, on the modulation of DL-{alpha}-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)/quisqualate receptor by phospholipase A{sub 2} (PLA{sub 2}), and on the formation of long-term potentiation (LTP) were studied in hippocampal membranes and hippocampal slices. Systemic administration of KA produced specific changes in the binding properties of the AMPA/quisqualate receptors and its regulation. Whereas the binding of various ligands to the N-methyl-D-aspartate receptors was not modified by KA treatment, there was a singificant decrease in the maximal number of binding sites for ({sup 3}H)AMPA. The loss of LTP was not due to changes in postsynaptic responses elicited by the bursts that trigger the potentiation effect, thus suggesting that KA treatment disrupts processes that follow N-methyl-D-aspartate receptor activation. Systemic administration of KA was associated with calpain activation as the amount of spectrin breakdown products was increased severalfold in hippocampus but not in cerebellum. Pretreatment of telencephalic membranes with calpain greatly reduced the PLA{sub 2}-induced increase in ({sup 3}H)AMPA binding. The results provide evidence in favor of an essential role of PLA{sub 2} in the development of LTP and suggest that the order of activation of different calcium-dependent processes is critical formore » producing the final changes underlying LTP.« less

Authors:
;  [1]; ;  [2]
  1. Univ. of Southern California, Los Angeles (United States)
  2. Univ. of California, Irvine (United States)
Publication Date:
OSTI Identifier:
6144644
Resource Type:
Journal Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America; (United States)
Additional Journal Information:
Journal Volume: 88:5; Journal ID: ISSN 0027-8424
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; EPILEPSY; PATHOGENESIS; LIPASES; BIOLOGICAL EFFECTS; RECEPTORS; BIOCHEMICAL REACTION KINETICS; GLUTAMIC ACID; HIPPOCAMPUS; PROPIONIC ACID; TRITIUM COMPOUNDS; AMINO ACIDS; BODY; BRAIN; CARBOXYLESTERASES; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM; DISEASES; ENZYMES; ESTERASES; HYDROGEN COMPOUNDS; HYDROLASES; KINETICS; MEMBRANE PROTEINS; MONOCARBOXYLIC ACIDS; NERVOUS SYSTEM; NERVOUS SYSTEM DISEASES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PROTEINS; REACTION KINETICS; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Massicotte, G, Baudry, M, Vanderklish, P, and Lynch, G. Modulation of DL-. alpha. -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid/quisqualate receptors by phospholipase A sub 2 : A necessary step in long-term potentiation. United States: N. p., 1991. Web. doi:10.1073/pnas.88.5.1893.
Massicotte, G, Baudry, M, Vanderklish, P, & Lynch, G. Modulation of DL-. alpha. -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid/quisqualate receptors by phospholipase A sub 2 : A necessary step in long-term potentiation. United States. https://doi.org/10.1073/pnas.88.5.1893
Massicotte, G, Baudry, M, Vanderklish, P, and Lynch, G. 1991. "Modulation of DL-. alpha. -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid/quisqualate receptors by phospholipase A sub 2 : A necessary step in long-term potentiation". United States. https://doi.org/10.1073/pnas.88.5.1893.
@article{osti_6144644,
title = {Modulation of DL-. alpha. -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid/quisqualate receptors by phospholipase A sub 2 : A necessary step in long-term potentiation},
author = {Massicotte, G and Baudry, M and Vanderklish, P and Lynch, G},
abstractNote = {The effects of kainate (KA)-induced epileptic seizures on the binding properites of hippocampal glutamate receptors, on the modulation of DL-{alpha}-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)/quisqualate receptor by phospholipase A{sub 2} (PLA{sub 2}), and on the formation of long-term potentiation (LTP) were studied in hippocampal membranes and hippocampal slices. Systemic administration of KA produced specific changes in the binding properties of the AMPA/quisqualate receptors and its regulation. Whereas the binding of various ligands to the N-methyl-D-aspartate receptors was not modified by KA treatment, there was a singificant decrease in the maximal number of binding sites for ({sup 3}H)AMPA. The loss of LTP was not due to changes in postsynaptic responses elicited by the bursts that trigger the potentiation effect, thus suggesting that KA treatment disrupts processes that follow N-methyl-D-aspartate receptor activation. Systemic administration of KA was associated with calpain activation as the amount of spectrin breakdown products was increased severalfold in hippocampus but not in cerebellum. Pretreatment of telencephalic membranes with calpain greatly reduced the PLA{sub 2}-induced increase in ({sup 3}H)AMPA binding. The results provide evidence in favor of an essential role of PLA{sub 2} in the development of LTP and suggest that the order of activation of different calcium-dependent processes is critical for producing the final changes underlying LTP.},
doi = {10.1073/pnas.88.5.1893},
url = {https://www.osti.gov/biblio/6144644}, journal = {Proceedings of the National Academy of Sciences of the United States of America; (United States)},
issn = {0027-8424},
number = ,
volume = 88:5,
place = {United States},
year = {Fri Mar 01 00:00:00 EST 1991},
month = {Fri Mar 01 00:00:00 EST 1991}
}