Effects of smoke inhalation on surfactant phospholipids and phospholipase A2 activity in the mouse lung

Journal Article · Tue Jan 01 00:00:00 EST 1991 · American Journal of Pathology; (USA)

OSTI ID:6143138

Oulton, M; Moores, H K; Scott, J E; Janigan, D T; Hajela, R ^[1]

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1. Dalhousie Univ., Halifax, Nova Scotia (Canada)

The effects of smoke inhalation on the pulmonary surfactant system were examined in mice exposed for 30 minutes to smoke generated from the burning of polyurethane foam. At 8 or 12 hours after exposure, surfactants were isolated separately from lung lavage (extracellular surfactant) and residual lung tissue (intracellular surfactant) for phospholipid analysis. Calcium-dependent phospholipase A2 (PLA2) was measured on a microsomal fraction prepared from the tissue homogenate. Smoke inhalation produced a twofold increase in extracellular surfactant total phospholipid. While there was no change in the total phospholipid or phosphatidylcholine (PC) content of the intracellular surfactant, smoke inhalation significantly decreased the disaturated species of PC (DSPC). The specific activity of PLA2 was reduced by more than 50% in both groups of exposed mice. Smoke inhalation appears to result in selective depletion of the DSPC of intracellular surfactant and PLA2 involved in its synthesis. This depletion may be compensated for by increased secretion or slower breakdown of the material present in the extracellular compartment.

• OSTI ID: 6143138
• Journal Information: American Journal of Pathology; (USA), Vol. 138:1; ISSN 0002-9440
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LIPASES
ENZYME ACTIVITY
PHOSPHOLIPIDS
BIOSYNTHESIS
POLYURETHANES
BIOLOGICAL EFFECTS
COMBUSTION
INHALATION
LUNGS
MICE
MICROSOMES
SECRETION
SMOKES
AEROSOLS
ANIMALS
BODY
CARBOXYLESTERASES
CELL CONSTITUENTS
CHEMICAL REACTIONS
COLLOIDS
DISPERSIONS
ENZYMES
ESTERASES
ESTERS
HYDROLASES
INTAKE
LIPIDS
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANIC POLYMERS
ORGANOIDS
ORGANS
OXIDATION
PETROCHEMICALS
PETROLEUM PRODUCTS
PLASTICS
POLYAMIDES
POLYMERS
RESIDUES
RESPIRATORY SYSTEM
RIBOSOMES
RODENTS
SOLS
SYNTHESIS
SYNTHETIC MATERIALS
THERMOCHEMICAL PROCESSES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology